Higashi Nobuyuki, Narimatsu Kiyoshiro, Okumura Minori, Nishimura Shin-Nosuke, Koga Tomoyuki
Department of Molecular Chemistry and Biochemistry, Faculty of Science and Engineering, Doshisha University, Kyotanabe, Kyoto 610-0321, Japan.
ACS Omega. 2019 May 2;4(5):8104-8111. doi: 10.1021/acsomega.9b00899. eCollection 2019 May 31.
Novel polymeric nanoparticles (NPs) with uniform sizes were prepared from peptide-vinyl polymer diblock hybrids by the self-organized precipitation method. Hybrid polymers of polystyrene (PSt) and tetrapeptide (cell-binding epitope RGDS, reverse SDGR, cationic KKKK, and anionic DDDD) were successfully synthesized by combining solid-phase peptide synthesis and reversible addition fragmentation chain transfer polymerization methods. Narrowly dispersed hybrid polymers (polydispersity index < 1.25, 14 000-17 000) were obtained. Altering the preparation conditions easily tuned the size and size distribution of the NPs. When the ζ-potentials for the NP suspensions were measured at pH 6.0, the obtained values corresponded to the net charge of each peptide segment. More importantly, the NPs could encapsulate fluorescent Nile red (NR) and magnetic iron oxide NP (MNP), which might be suitable for fluorescent imaging and magnet-induced patterning of cells, respectively. The interactions of NPs with cells (NIH/3T3 fibroblast) and the magnetic effects were examined for NR/MNP-loaded PSt-RGDS and -SDGR NPs. Both NPs were readily incorporated into cells, but only NR/MNP-loaded PSt-RGDS NP showed magnetic responsiveness in cell adhesion and cultures.
通过自组装沉淀法，由肽 - 乙烯基聚合物二嵌段杂化物制备了尺寸均匀的新型聚合物纳米颗粒（NPs）。通过将固相肽合成法和可逆加成 - 断裂链转移聚合法相结合，成功合成了聚苯乙烯（PSt）与四肽（细胞结合表位RGDS、反向SDGR、阳离子KKKK和阴离子DDDD）的杂化聚合物。获得了窄分布的杂化聚合物（多分散指数<1.25，14 000 - 17 000）。改变制备条件可轻松调节纳米颗粒的尺寸和尺寸分布。当在pH 6.0下测量NP悬浮液的ζ电位时，所得值与每个肽段的净电荷相对应。更重要的是，纳米颗粒可以包封荧光尼罗红（NR）和磁性氧化铁纳米颗粒（MNP），它们可能分别适用于细胞的荧光成像和磁诱导图案化。研究了载有NR/MNP的PSt - RGDS和 - SDGR纳米颗粒与细胞（NIH/3T3成纤维细胞）的相互作用以及磁效应。两种纳米颗粒都很容易被细胞摄取，但只有载有NR/MNP的PSt - RGDS纳米颗粒在细胞粘附和培养中表现出磁响应性。
