Campbell Thomas D, Febrian Rio, Kleinschmidt Holly E, Smith Kevin A, Bracher Paul J
Department of Chemistry, Saint Louis University, 3501 Laclede Avenue, St. Louis, Missouri 63103 United States.
ACS Omega. 2019 Jul 26;4(7):12745-12752. doi: 10.1021/acsomega.9b01492. eCollection 2019 Jul 31.
This paper describes a method for the quantitative analysis of mixtures of glycine and its oligomers by ion-pair high-performance liquid chromatography (IP-HPLC), with a particular focus on applications in origins-of-life research. We demonstrate the identification of glycine oligomers (Gly ) up to 14 residues long-the approximate detectable limit of their solubility in water-and measurement of the concentration of these species in the product mixture of an oligomerization reaction. The molar response factors for higher oligomers of glycine-which are impractical to obtain as pure samples-are extrapolated from direct analysis of pure standards of = 3-6, which established a clear linear trend. We compare and contrast our method to those in previous reports with respect to accuracy and practicality. While the data reported here are specific to the analysis of oligomers of glycine, the approach should be applicable to the design of methods for the analysis of oligomerization of other amino acids.
本文描述了一种通过离子对高效液相色谱法（IP-HPLC）对甘氨酸及其低聚物混合物进行定量分析的方法，特别关注其在生命起源研究中的应用。我们展示了对长达14个残基的甘氨酸低聚物（Gly ）的鉴定——这是它们在水中溶解度的近似可检测极限——以及对低聚反应产物混合物中这些物质浓度的测量。甘氨酸更高低聚物的摩尔响应因子——作为纯样品难以获得——是从对 = 3-6的纯标准品的直接分析中外推得到的，这建立了一个明显的线性趋势。我们在准确性和实用性方面将我们的方法与先前报告中的方法进行了比较和对比。虽然这里报告的数据特定于甘氨酸低聚物的分析，但该方法应适用于其他氨基酸低聚分析方法的设计。
