Geravand Maryam, Fallah Parviz, Yaghoobi Mojtaba Hedayat, Soleimanifar Fatemeh, Farid Malihe, Zinatizadeh Nazi, Yaslianifard Somayeh
Alborz University of Medical Sciences, School of Medicine, Student Research Committee, Karaj, Iran.
Alborz University of Medical Sciences, School of Paramedical Sciences, Department of Hematology, Karaj, Iran.
Arq Gastroenterol. 2019 Aug 13;56(2):141-145. doi: 10.1590/S0004-2803.201900000-28.
BACKGROUND: Colorectal cancer is one of the most commonly diagnosed cancers around the world. One of the factors involved in the development of colorectal cancer is the changes in the normal flora of the intestine. OBJECTIVE: In this study, the mean copy number of Enterococcus faecalis in people with polyps and people with colorectal cancer has been evaluated in comparison with healthy controls. METHODS: In this study, 25 patients with colorectal cancer and 28 patients with intestinal polyps were selected and stool specimens were taken. In addition, 24 healthy individuals were selected as control group. Extraction of bacterial DNA from the stool sample were performed. The molecular methods of PCR for confirmation of standard strain and absolute Real Time PCR (qRT-PCR) method were used to evaluate the number of Enterococcus faecalis in the studied groups. RESULTS: The results of this study indicate that the mean copy number of Enterococcus faecalis in patients with colorectal cancer was 11.2x109 per gram of stool, and in patients with polyps was 9.4x108 per gram of stool. In healthy people, this number was 9x108 per gram of stool. There was a significant difference between the implicit copy numbers in the three groups. (P<0.05). CONCLUSION: Enterococcus faecalis in faecal flora of people with colorectal cancer was significantly higher than those with polyps and healthy people. This could potentially signify the ability of this bacterium to induce colorectal cancer. More studies are needed to prove this theory.
背景：结直肠癌是全球最常见的诊断癌症之一。肠道正常菌群的变化是结直肠癌发生发展的因素之一。 目的：在本研究中，评估了息肉患者和结直肠癌患者粪便中粪肠球菌的平均拷贝数，并与健康对照进行比较。 方法：本研究选取了25例结直肠癌患者和28例肠道息肉患者，采集粪便标本。此外，选取24名健康个体作为对照组。从粪便样本中提取细菌DNA。采用PCR分子方法鉴定标准菌株，并使用绝对实时荧光定量PCR（qRT-PCR）方法评估研究组中粪肠球菌的数量。 结果：本研究结果表明，结直肠癌患者粪便中粪肠球菌的平均拷贝数为每克粪便11.2×10⁹，息肉患者为每克粪便9.4×10⁸。在健康人中，这一数字为每克粪便9×10⁸。三组的隐含拷贝数之间存在显著差异（P<0.05）。 结论：结直肠癌患者粪便菌群中的粪肠球菌显著高于息肉患者和健康人。这可能意味着这种细菌具有诱发结直肠癌的能力。需要更多研究来证实这一理论。
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