Füssle R, Sziegoleit A
Institute of Medical Microbiology, University of Giessen.
Zentralbl Bakteriol Mikrobiol Hyg A. 1988 Nov;269(3):346-54. doi: 10.1016/s0176-6724(88)80178-0.
The incorporation of staphylococcal alpha-toxin into glutaraldehyde fixed erythrocytes occurs in the same way as with native erythrocytes. Binding of alpha-toxin to the cells is accompanied by oligomerization of native 3 S toxin to the membrane-bound 11 S toxin hexamer, which is embedded into the lipid bilayer of the membrane. Antibodies against alpha-toxin, build up during an infection with S. aureus, can be determined in a passive hemagglutination test (IHT) using glutaraldehyde fixed and alpha-toxin treated erythrocytes. To test the validity of this IHT, antibodies to alpha-toxin were determined in 550 human sera of patients from hospitals of the University of Giessen suspected to suffer from staphylococcal infections and in 300 sera of healthy blood donors. The results were compared with the titres obtained by a convenient neutralisation test (ASTA). All sera with elevated titres in the ASTA test also showed high titres in the IHT. Because it is simple to perform and highly reproducible, the IHT seems to be a valuable test for detection of antibodies against staphylococcal alpha-toxin.
金黄色葡萄球菌α毒素掺入戊二醛固定的红细胞的方式与天然红细胞相同。α毒素与细胞的结合伴随着天然3S毒素寡聚化为膜结合的11S毒素六聚体，该六聚体嵌入细胞膜的脂质双层中。在金黄色葡萄球菌感染期间产生的抗α毒素抗体，可以使用戊二醛固定并经α毒素处理的红细胞，通过被动血凝试验（IHT）来测定。为了检验该IHT的有效性，在吉森大学医院疑似患有葡萄球菌感染的550例患者的人血清和300例健康献血者的血清中，测定了抗α毒素抗体。将结果与通过便捷中和试验（ASTA）获得的滴度进行比较。在ASTA试验中滴度升高的所有血清在IHT中也显示出高滴度。由于IHT操作简单且具有高度可重复性，它似乎是检测抗葡萄球菌α毒素抗体的一种有价值的试验。
