Alshawa Eiman, Laqqan Mohammed, Montenarh Mathias, Hammadeh Mohamad Eid
Department of Obstetrics, Gynecology & Assisted Reproduction Laboratory, Saarland University, Homburg, Germany.
Medical Biochemistry and Molecular Biology, Saarland University, Homburg, Germany.
Toxicol Rep. 2019 Aug 6;6:819-824. doi: 10.1016/j.toxrep.2019.08.004. eCollection 2019.
This study designed to assess the expression level of and to evaluate the levels of CatSper2 and Tektin2 proteins in human spermatozoa before and after cryopreservation. One hundred and twenty semen samples were included in this study. All the samples were subjected to qPCR and Western blot analysis. The results showed a significant reduction in the expression levels of and in the cryopreserved compared to the fresh samples (P = 0.0039 and P = 0.0166, respectively), and the results showed down-regulation in the expression level of genes between the study groups. Moreover, the protein levels of the CatSper2 and Tektin2 were lower in cryopreserved samples compared to fresh samples (P = 0.0001). In conclusion, the reduction in the proteins level and expression level of the 2 and 2 in cryopreserved samples could be used as an indicator of sperm motility loss.
本研究旨在评估[未提及具体基因名称]的表达水平，并评价冷冻保存前后人精子中CatSper2和Tektin2蛋白的水平。本研究纳入了120份精液样本。所有样本均进行了qPCR和蛋白质印迹分析。结果显示，与新鲜样本相比，冷冻保存样本中[未提及具体基因名称]的表达水平显著降低（分别为P = 0.0039和P = 0.0166），且研究组之间[未提及具体基因名称]基因的表达水平下调。此外，与新鲜样本相比，冷冻保存样本中CatSper2和Tektin2的蛋白水平较低（P = 0.0001）。总之，冷冻保存样本中2和2的蛋白水平和表达水平降低可作为精子活力丧失的指标。
