Winton D J, Flaks B
Department of Pathology, University of Bristol, Medical School, UK.
Br J Exp Pathol. 1988 Dec;69(6):877-89.
A method for the quantitative analysis of the degree to which hepatocyte rough endoplasmic reticulum (ER) is arranged into parallel arrays was used to study the effect of fasting on rough ER aggregation in rat liver cells following either bilateral adrenalectomy or administration of the carcinogenic azo dye 3'-methyl-4-dimethylaminoazobenzene (3'MeDAB). One group of male inbred Leeds strain rats was subjected to bilateral adrenalectomy (ADX): after 1 week half of the animals were fasted for 24 h whereupon the whole group was killed. A second group of rats, fed for 4 weeks on a diet containing 0.06% of the carcinogenic azo dye 3'MeDAB, was similarly divided into two groups that were killed either with or without a prior 24-h fast. Untreated control groups of rats, both fasted and unfasted, were also killed. A quantitative electron microscope study was carried out to investigate the effect of each treatment on the degree to which the hepatocyte rough ER was aggregated into parallel arrays. ADX alone was without effect but caused a dramatic fall in rough ER aggregation when combined with fasting. At least as great an effect was induced by 3'MeDAB, with or without fasting, while fasting alone had a significant but much more modest effect than either ADX or the carcinogen. Thus, two disparate treatments induced morphologically identical responses in hepatocyte rough ER. The implications of this are discussed in terms of known interrelations between glucocorticoids and chemical carcinogenesis in the rat liver.
一种用于定量分析肝细胞粗面内质网（ER）排列成平行阵列程度的方法，被用于研究禁食对大鼠肝细胞粗面内质网聚集的影响，这些大鼠在双侧肾上腺切除或给予致癌偶氮染料3'-甲基-4-二甲基氨基偶氮苯（3'MeDAB）后出现这种情况。一组雄性近交利兹品系大鼠接受双侧肾上腺切除（ADX）：1周后，其中一半动物禁食24小时，然后将整个组处死。第二组大鼠，用含0.06%致癌偶氮染料3'MeDAB的饲料喂养4周，同样分为两组，一组在禁食24小时后处死，另一组不禁食处死。未处理的禁食和未禁食大鼠对照组也被处死。进行了定量电子显微镜研究，以调查每种处理对肝细胞粗面内质网聚集成平行阵列程度的影响。单独的ADX没有效果，但与禁食结合时会导致粗面内质网聚集显著下降。无论是否禁食，3'MeDAB诱导的效果至少同样大，而单独禁食的效果显著，但比ADX或致癌物的效果要小得多。因此，两种不同的处理在肝细胞粗面内质网中诱导出形态相同的反应。根据已知的大鼠肝脏中糖皮质激素与化学致癌作用之间的相互关系，对这一现象的意义进行了讨论。
