Blunck J M, Smith M L, Vorrath E M
Res Commun Chem Pathol Pharmacol. 1982 Nov;38(2):255-69.
The DNA and RNA content and the mitotic index were determined in the livers of rats pair-fed for 10 days on a control diet, and diets containing 0.06% 3'-methyl-4-dimethylaminoazobenzene (3'MeDAB), either alone or in combination with the inhibitors of azo dye carcinogenesis acetanilide (0.08%), p-hydroxypropiophenone (PHP, 1%), urea (5%), dehydroacetic acid (DHAA, 0.25%), butylated hydroxytoluene (BHT, 0.3%) and 3-methylcholanthrene (3MC, 0.067%). The liver RNA/DNA ratio was increased in rats fed the 3'MeDAB diet following ingestion of each of the inhibitor plus carcinogen-containing diets. The increases were significant except in the instance of urea. The increase was mainly due to increased RNA content in rats fed 3'MeDAB plus acetanilide, DHAA, BHT and 3MC when compared with rats fed 3'MeDAB alone. In rats fed PHP and 3'MeDAB, an increase in total liver DNA that was noted in rats fed 3'MeDAB alone did not take place, while total liver RNA content was similar in both groups; the RNA/DNA ratio was therefore greater in rats protected from 3'MeDAB carcinogenesis by PHP. The results are discussed in relationship to the effects of carcinogens on RNA forming genes and the role of the nucleolus in cell differentiation.
在对大鼠进行配对喂养的实验中，测定了对照组饮食以及分别含有0.06% 3'-甲基-4-二甲基氨基偶氮苯（3'MeDAB）的饮食，其中3'MeDAB饮食又分为单独含3'MeDAB组，以及3'MeDAB与偶氮染料致癌作用抑制剂乙酰苯胺（0.08%）、对羟基苯丙酮（PHP，1%）、尿素（5%）、脱氢乙酸（DHAA，0.25%）、丁基化羟基甲苯（BHT，0.3%）和3-甲基胆蒽（3MC，0.067%）联合使用的各组大鼠肝脏中的DNA和RNA含量以及有丝分裂指数。在摄入每种抑制剂加致癌物的饮食后，喂食3'MeDAB饮食的大鼠肝脏RNA/DNA比值升高。除尿素组外，其他组的升高均具有显著性。与单独喂食3'MeDAB的大鼠相比，喂食3'MeDAB加乙酰苯胺、DHAA、BHT和3MC的大鼠RNA含量增加，这是导致比值升高的主要原因。在喂食PHP和3'MeDAB的大鼠中，单独喂食3'MeDAB的大鼠肝脏总DNA增加的情况未出现，而两组肝脏总RNA含量相似；因此，PHP对3'MeDAB致癌作用有保护作用的大鼠其RNA/DNA比值更高。结合致癌物对RNA形成基因的影响以及核仁在细胞分化中的作用对结果进行了讨论。
