Cavill I, Trevett D, Fisher J, Hoy T
Department of Haematology, University of Wales College of Medicine, Cardiff.
Br J Haematol. 1988 Dec;70(4):491-3. doi: 10.1111/j.1365-2141.1988.tb02522.x.
Present methods for measuring red cell volume are based on the dilution of radioactively labelled cells. This precludes the investigation in neonates and pregnant women. We present a simple method for labelling red cells with biotin. These cells may be injected intravenously and subsequently detected using streptavidin-FITC and flow-cytometry. A comparison of the red cell volume estimated using both 51Cr and biotin labelled cells in 19 patients showed no consistent clinically significant difference between the two. This novel label appears to allow red volume to be reliably estimated without using radioactivity.
目前测量红细胞体积的方法是基于对放射性标记细胞的稀释。这排除了对新生儿和孕妇的研究。我们提出了一种用生物素标记红细胞的简单方法。这些细胞可以静脉注射，随后使用链霉亲和素-异硫氰酸荧光素和流式细胞术进行检测。对19例患者使用51Cr和生物素标记细胞估算的红细胞体积进行比较，结果显示两者之间没有一致的具有临床意义的显著差异。这种新型标记似乎可以在不使用放射性的情况下可靠地估算红细胞体积。
