Hoffmann-Fezer G, Mysliwietz J, Mörtlbauer W, Zeitler H J, Eberle E, Hönle U, Thierfelder S
Institut für Immunologie, GSF, München, Germany.
Ann Hematol. 1993 Aug;67(2):81-7. doi: 10.1007/BF01788131.
Biotin labeling of red cells was studied using different approaches to see if biotinylation is a useful label for determination of erythrocyte survival. Mouse red cells were labeled with biotin, either in vivo by injection or in vitro. In vivo labeled red cells were followed up in some mice without transfusing the labeled erythrocytes. Furthermore, in vivo labeled as well as in vitro labeled red cells were transfused into syngeneic mice. The biotin label allows an easy discrimination between labeled and unlabeled red cells during FACS analysis, and it is relatively stable for at least 50 days. All the three different approaches give similar results. Mean red cell life spans of in vivo or in vitro labeled red cells either transfused or followed up in vivo were between 44 and 52 days (T50 mean 23.9 days) when red cell destruction was assumed to be only a result of senescence. Mean red cell life spans were between 8 and 18 days (T50 mean 9.5 days) when a random destruction independent of red cell age was suggested. All the survival slopes are neither simple linear functions of time nor logarithmic functions, but they show an overlay of linear function by a logarithmic function where the components of both are unknown.
采用不同方法研究了红细胞的生物素标记，以确定生物素化是否是用于测定红细胞存活的有效标记。小鼠红细胞通过注射在体内或在体外进行生物素标记。在一些小鼠中对体内标记的红细胞进行追踪，而不输入标记的红细胞。此外，将体内标记和体外标记的红细胞输注到同基因小鼠体内。生物素标记在流式细胞术分析期间能够轻松区分标记和未标记的红细胞，并且至少50天内相对稳定。所有这三种不同方法都给出了相似的结果。当假定红细胞破坏仅是衰老的结果时，体内或体外标记的红细胞在体内输注或追踪时的平均红细胞寿命在44至52天之间（T50平均为23.9天）。当提示存在与红细胞年龄无关的随机破坏时，平均红细胞寿命在8至18天之间（T50平均为9.5天）。所有存活斜率既不是时间的简单线性函数也不是对数函数，而是显示出对数函数与线性函数的叠加，其中两者的成分均未知。
