Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand.
Japan Collection of Microorganisms, RIKEN BioResource Center, Ibaraki, 305-0074, Japan.
Arch Microbiol. 2020 Jan;202(1):55-61. doi: 10.1007/s00203-019-01717-w. Epub 2019 Aug 28.
A novel actinomycete, strain PA1-10, isolated from the leaf of Phyllanthus amarus collected from Bangkok, Thailand, was characterized taxonomically using a polyphasic approach. This strain contained the characteristics consistent with those of members of the genus Nonomuraea. It formed short rugose spore chain on aerial mycelium. The diamino acid in cell wall peptidoglycan was meso-diaminopimelic acid. Galactose, glucose, madurose, mannose, and ribose were found in whole-cell hydrolysates. Predominant menaquinones were MK-9 (H), MK-9 (H), and MK-9 (H). Major cellular fatty acids were iso-C and C 10-methyl. Phospholipid profiles were composed of phosphatidylinositol mannoside (PIM), lyso-phosphatidylethanolamine (lyso-PE), phosphatidylethanolamine (PE), methylphosphatidylethanolamine (PME), diphosphatidylglycerol (DPG), and phosphatidylglycerol (PG). The G + C content of DNA was 71.2 mol%. Strain PA1-10 showed the highest 16S rRNA gene sequence similarity with Nonomuraea candida JCM 15928 (98.35%) and shared the same node with Nonomuraea maritima JCM 18321 in the phylogenetic tree analysis. Based on the phenotypic characteristics, DNA-DNA relatedness, and average nucleotide identity (ANI), the strain is considered to represent a novel species of the genus Nonomuraea, for which the name Nonomuraea phyllanthi is proposed. The type strain is PA1-10 (= JCM 33073 = NBRC 112774 = TISTR 2497).
一株新型放线菌，菌株 PA1-10，从泰国曼谷采集的叶状叶下珠中分离出来，采用多相分类法进行了分类学特征描述。该菌株的特征与野野村菌属的成员一致。它在气生菌丝上形成短而粗糙的孢子链。细胞壁肽聚糖中的二氨基氨基酸是 meso-二氨基庚二酸。在全细胞水解物中发现了半乳糖、葡萄糖、甘露糖、甘露糖和核糖。主要的menaquinones 是 MK-9 (H)、MK-9 (H) 和 MK-9 (H)。主要的细胞脂肪酸是 iso-C 和 C 10-甲基。磷脂图谱由磷脂酰肌醇甘露糖苷（PIM）、溶血磷脂酰乙醇胺（lyso-PE）、磷脂酰乙醇胺（PE）、甲基磷脂酰乙醇胺（PME）、双磷脂酰甘油（DPG）和磷脂酰甘油（PG）组成。DNA 的 G+C 含量为 71.2 mol%。菌株 PA1-10 与野野村菌 Nonomuraea candida JCM 15928 的 16S rRNA 基因序列相似度最高（98.35%），并在系统发育树分析中与野野村菌 Nonomuraea maritima JCM 18321 处于同一分支。基于表型特征、DNA-DNA 相关性和平均核苷酸同一性（ANI），该菌株被认为代表野野村菌属的一个新种，命名为 Phyllanthi nomonuraea。模式菌株为 PA1-10（=JCM 33073=TISTR 2497）。
