Sagar Sharath V, Saini Kuldeep, Sharma Anil Kumar, Kumar Sachin, Kumar Rinesh, Fular Ashutosh, Shakya Mukesh, Upadhaya Deepak, Nagar Gaurav, C Shanmuganath, Samanta Subhamoy, Kumar Suman, Ghosh Srikant
Entomology Laboratory, Division of Parasitology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India.
Department of Parasitology, College of Veterinary Science and Animal Husbandry, Rewa, Madhya Pradesh, India.
Trop Anim Health Prod. 2020 Mar;52(2):611-618. doi: 10.1007/s11250-019-02048-0. Epub 2019 Aug 28.
The isolates of Rhipicephalus microplus collected from Madhya Pradesh (MP), Punjab (PJB) and Uttar Pradesh (UP) states of India were characterized using laboratory standardized adult immersion test (AIT) against macrocyclic lactone (ivermectin), synthetic pyrethroids (cypermethrin and deltamethrin), organophosphates (coumaphos and diazinon) and phenylpyrazole compounds (fipronil). Out of the six isolates tested, five isolates except MTH were resistant to deltamethrin and cypermethrin at level II with RF ranging from 16.4 to 24.02 and 7.05 to 13.2, respectively. In case of organophosphates, coumaphos was less effective showing resistance level II (RF 8.52-11.2) in all the six populations compared with diazinon to which three isolates (MHW, RWA and AGS) were resistant at level II. Except MTH, other five isolates were categorized at level I with RF ranging from 1.53 to 3.02 against ivermectin. The phenylpyrazole compound however was found effective, and none of the isolates could survive at a discriminating concentration. The possible strategy for the management of multi-acaricide-resistant ticks in the surveyed districts was discussed in the present study.
从印度中央邦（MP）、旁遮普邦（PJB）和北方邦（UP）采集的微小扇头蜱分离株，采用实验室标准化的成虫浸泡试验（AIT），针对大环内酯类（伊维菌素）、合成拟除虫菊酯类（氯氰菊酯和溴氰菊酯）、有机磷类（蝇毒磷和二嗪农）以及苯基吡唑类化合物（氟虫腈）进行特性鉴定。在测试的6个分离株中，除MTH外，有5个分离株对溴氰菊酯和氯氰菊酯呈II级抗性，抗性倍数分别为16.4至24.02和7.05至13.2。在有机磷类方面，蝇毒磷效果较差，所有6个种群均表现为II级抗性（抗性倍数8.52 - 11.2），而二嗪农有3个分离株（MHW、RWA和AGS）呈II级抗性。除MTH外，其他5个分离株对伊维菌素呈I级抗性，抗性倍数为1.53至3.02。然而，苯基吡唑类化合物被发现有效，在鉴别浓度下没有分离株能够存活。本研究讨论了在所调查地区管理多杀螨剂抗性蜱的可能策略。
