Valencia Echavarria Diana Marcela, Giraldo Valderrama Luis Alfonso, Marín Gómez Alejandra
Centro de Investigación el Nus- San Roque, Corporación Colombiana de Investigación Agropecuaria-AGROSAVIA, San Roque, Colombia.
Facultad de Ciencias Agrarias, Departamento de Producción Animal, Universidad Nacional de Colombia, Sede Medellín, Medellín, Colombia.
Trop Anim Health Prod. 2020 Mar;52(2):565-571. doi: 10.1007/s11250-019-02043-5. Epub 2019 Aug 29.
The aim of this trial was to determine the effect of ruminal fluid adapted to crude glycerol supplementation on in vitro methane and fermentation parameters of the forage Pennisetum clandestinum Hochst. Ex Chiov (Kikuyu forage) of 35 (D35) and 45 days (D45) of regrowth. Four non-lactating cannulated Holstein cows with 600 ± 33 kg LW, under a randomized Latin square design (4 animals × 4 periods), were used to obtain ruminal fluid after adaptation for 13 days within each period to supplementation in the rumen with crude glycerol (GL): 0 (GL0), 500 (GL1), 1000 (GL2), and 1500 g/cow/day (GL3). All the variables were analyzed under a 4 × 2 split-plot arrangement, where the ruminal fluid adaptation to supplementation with GL and its interaction with the incubated diets (GL× D) were analyzed. Ruminal pH values and in vitro apparent degradability of the dry matter (DMd) and organic matter (OMd) were not affected, with a decrease in the concentrations of NH-N and acetate by the ruminal fluid adapted to crude glycerol. The concentrations of propionate and butyrate increased, without affecting the total concentration of VFA in the ruminal fluid. The in vitro methane production increased with forage D35 and GL1, GL2, GL3. The in vitro methane production with the D45 forage increased with the ruminal fluid adapted to GL2 and GL3. The ruminal fluid adapted to crude glycerol supplementation increased in vitro methane production when incubating the forage Kikuyu.
本试验的目的是确定适应添加粗甘油的瘤胃液对生长35天（D35）和45天（D45）的狼尾草（Chiov.隐花狼尾草，即菊芋牧草）体外甲烷和发酵参数的影响。采用随机拉丁方设计（4头动物×4个时期），选用4头体重600±33千克的非泌乳带瘘管荷斯坦奶牛，在每个时期内经过13天适应期后，在瘤胃中添加粗甘油（GL）：0（GL0）、500（GL1）、1000（GL2）和1500克/头/天（GL3），以获取瘤胃液。所有变量均采用4×2裂区设计进行分析，分析瘤胃液对添加GL的适应性及其与培养日粮的相互作用（GL×D）。瘤胃pH值以及干物质（DMd）和有机物质（OMd）的体外表观降解率不受影响，适应粗甘油的瘤胃液使NH-N和乙酸盐浓度降低。丙酸盐和丁酸盐浓度增加，但不影响瘤胃液中挥发性脂肪酸（VFA）的总浓度。对于D35的牧草，体外甲烷产量随GL1、GL2、GL3增加。对于D45的牧草，体外甲烷产量随适应GL2和GL3的瘤胃液增加。在培养菊芋牧草时，适应添加粗甘油的瘤胃液可提高体外甲烷产量。
