University Institute of Clinical Chemistry, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.
Department of Diabetes, Endocrinology, Clinical Nutrition & Metabolism, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.
Metabolomics. 2019 Aug 28;15(9):120. doi: 10.1007/s11306-019-1585-3.
Non-targeted metabolic profiling using high-resolution mass spectrometry (HRMS) is a standard approach for pathway identification despite technical limitations.
To assess the performance of combining targeted quadrupole (QQQ) analysis with HRMS for in-depth pathway profiling.
Serum of exercising patients with type 1 diabetes (T1D) was profiled using targeted and non-targeted assays.
Non-targeted analysis yielded a broad unbiased metabolic profile, targeted analysis increased coverage of purine metabolism (twofold) and TCA cycle (three metabolites).
Our screening strategy combined the benefits of the unbiased full-scan HRMS acquisition with the deeper insight into specific pathways by large-scale QQQ analysis.
尽管存在技术限制，但使用高分辨率质谱（HRMS）进行非靶向代谢组学分析是一种标准的通路鉴定方法。
评估将靶向四极杆（QQQ）分析与 HRMS 相结合进行深入通路分析的性能。
使用靶向和非靶向分析对 1 型糖尿病（T1D）患者进行运动后的血清进行分析。
非靶向分析产生了广泛的无偏代谢谱，靶向分析增加了嘌呤代谢（两倍）和 TCA 循环（三种代谢物）的覆盖范围。
我们的筛选策略结合了 HRMS 全扫描无偏采集的优势，以及通过大规模 QQQ 分析对特定通路的深入了解。
