School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, China.
Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, People's Republic of China.
Environ Sci Pollut Res Int. 2019 Oct;26(30):31234-31242. doi: 10.1007/s11356-019-06249-w. Epub 2019 Aug 28.
Yarrowia lipolytica (Y. lipolytica) is an oleaginous yeast that can utilize hydrophobic substrates as carbon source to produce single-cell lipids for biodiesel production. This study attempts to increase the lipid accumulation ability of Y. lipolytica by first gradually elevating pure oil substrate concentration during the cultivation and then adding short-chain carbon compounds, such as glucose and sodium acetate, to a culture substance according to the optimal oil concentration. Results showed that Y. lipolytica cultured under 40.0 g L oil concentration showed higher lipids (2.97 g L) and lipid content (37.35%, DW) compared with that cultured under 20.0 g L, where the corresponding values were 1.91 g L and 24.46%. By contrast, the lipid content of Y. lipolytica increased from 37.35 to 41.50% when the substrate was changed from 40.0 g L pure oil to 5% sodium acetate combined with 95% oil under the same total carbon concentration. However, lipid accumulation did not increase even though 15% sodium acetate or 5% glucose, or 15% glucose was added to the combined substrate. Moreover, the lipid biomodification of Y. lipolytica was evident when it was cultured under the oil concentration of 20.0 g L. Therefore, the lipid accumulation of Y. lipolytica can be elevated through the gradient increase of oil concentration and by adding a suitable amount of easily degradable carbon source. Furthermore, the lipid biomodification of Y. lipolytica improves biodiesel quality.
解脂耶氏酵母（Yarrowia lipolytica）是一种产油酵母，能够利用疏水性底物作为碳源生产生物柴油用单细胞油脂。本研究尝试通过在培养过程中逐渐提高纯油底物浓度，然后根据最佳油浓度向培养物中添加葡萄糖和乙酸钠等短链碳化合物，来提高解脂耶氏酵母的油脂积累能力。结果表明，在 40.0 g/L 油浓度下培养的解脂耶氏酵母的油脂（2.97 g/L）和油脂含量（37.35%，DW）均高于在 20.0 g/L 油浓度下培养的解脂耶氏酵母（分别为 1.91 g/L 和 24.46%）。相比之下，当底物由 40.0 g/L 纯油变为总碳浓度相同的 5%乙酸钠与 95%油的组合时，解脂耶氏酵母的油脂含量从 37.35%增加到 41.50%。然而，当在组合底物中添加 15%乙酸钠或 5%葡萄糖或 15%葡萄糖时，油脂积累并没有增加。此外，当在 20.0 g/L 的油浓度下培养解脂耶氏酵母时，其油脂的生物修饰作用明显。因此，通过逐渐提高油浓度和添加适量易降解的碳源，可以提高解脂耶氏酵母的油脂积累能力。此外，解脂耶氏酵母的油脂生物修饰作用可提高生物柴油的质量。
