使用RNAscope™原位杂交技术进行表达分析。

Expression Analysis by RNAscope™ In Situ Hybridization.

作者信息

Mead Timothy J, Apte Suneel S

机构信息

Department of Biomedical Engineering, Cleveland Clinic Lerner Research Institute, Cleveland, OH, USA.

Department of Biomedical Engineering-ND20, Cleveland Clinic Lerner Research Institute, Cleveland, OH, USA.

出版信息

Methods Mol Biol. 2020;2043:173-178. doi: 10.1007/978-1-4939-9698-8_14.

DOI:10.1007/978-1-4939-9698-8_14

PMID:31463911

Abstract

RNA in situ hybridization has an important place in matrix biology, as the only method that allows for in situ discrimination of precise spatial and temporal patterns of gene expression. Whereas immunohistochemistry shows where a matrix protein localizes, ISH identifies the cell of origin. Thus, these methods provide complementary information for insights on the life cycle of matrix molecules, including ADAMTS proteases. This protocol encompasses the staining of tissue sections to reveal expression of the gene of interest.

摘要

RNA原位杂交在基质生物学中占有重要地位，因为它是唯一能够在原位区分基因表达精确时空模式的方法。免疫组织化学显示基质蛋白的定位，而原位杂交则确定其起源细胞。因此，这些方法为深入了解包括ADAMTS蛋白酶在内的基质分子的生命周期提供了互补信息。本方案涵盖组织切片染色以揭示感兴趣基因的表达。