School of Environmental Science, Liaoning University, Shenyang, China.
State Key Laboratory of Urban Water Resources and Environment, School of Environment, Harbin Institute of Technology, Harbin, China.
Plant Biol (Stuttg). 2020 Jan;22(1):62-69. doi: 10.1111/plb.13039. Epub 2019 Sep 25.
Arbuscular mycorrhizal fungi (AMF) is an effective way to remove heavy metals' inhibition on plants, however, few relevant research attempts have been made to determine the contribution of AMF to the physiological and biochemical changes related to the enhanced copper tolerance of Phragmites australis under metal-stressed conditions. In this study, the effects of AMF inoculation on P. australis under different concentrations of copper stress were investigated according to the changes in the parameters related to growth and development, and photosynthetic charateristics. Then, differentially expressed proteins (DEPs) were evaluated by the Isobaric Tag for Relative and Absolute Quantification (iTRAQ) system, which could accurately quantify the DEPs by measuring peak intensities of reporter ions in tandem mass spectrometry (MS/MS) spectra. It was found that AMF inoculation may relieve the photosynthesis inhibition caused by copper stress on P. australis and thus promote growth. Proteomic analysis results showed that under copper stress, the inoculation of R. irregularis resulted in a total of 459 differently-expressed proteins (200 up-regulated and 259 down-regulated) in root buds. In addition, the photosynthetic changes caused by AMF inoculation mainly involve the up-regulated expression of transmembrane protein-pigment complexes CP43 (photosystem II) and FNR (ferredoxin-NADP+ oxidoreductase related to photosynthetic electron transport). These results indicate that AMF could effectively improve the growth and physiological activity of P. australis under copper stress, and thus provides a new direction and instructive evidence for determining the mechanisms by which AMF inoculation enhances the copper tolerance of plants.
丛枝菌根真菌（AMF）是一种有效去除重金属对植物抑制的方法，然而，很少有相关研究试图确定 AMF 对重金属胁迫条件下增强的芦苇铜耐受性相关的生理生化变化的贡献。在这项研究中，根据与生长和发育以及光合作用特性相关的参数变化，研究了 AMF 接种对不同铜胁迫浓度下芦苇的影响。然后，通过等重标记相对和绝对定量（iTRAQ）系统评估差异表达蛋白（DEPs），该系统通过测量串联质谱（MS/MS）谱中报告离子的峰强度可以准确地定量 DEPs。结果发现，AMF 接种可能缓解铜胁迫对芦苇光合作用的抑制，从而促进生长。蛋白质组学分析结果表明，在铜胁迫下，接种粗糙脉孢菌导致根芽中总共 459 种差异表达蛋白（200 种上调和 259 种下调）。此外，AMF 接种引起的光合作用变化主要涉及跨膜蛋白-色素复合物 CP43（光系统 II）和 FNR（与光合电子传递相关的铁氧还蛋白-NADP+氧化还原酶）的上调表达。这些结果表明，AMF 可以有效地提高铜胁迫下芦苇的生长和生理活性，为确定 AMF 接种增强植物铜耐受性的机制提供了新的方向和指导证据。
