Department of Orthopaedics, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.
Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, 310000, China.
Adv Healthc Mater. 2019 Oct;8(19):e1900831. doi: 10.1002/adhm.201900831. Epub 2019 Aug 28.
Tendon to bone (enthesis) rupture, which may cause disability and persistent pain, shows high rate of re-rupture after surgical repair. Tendon or enthesis scaffolds have been widely studied, but few of these materials can recapitulate the tissue continuity. Thus, this study is conducted to prepare a triphasic decellularized bone-fibrocartilage-tendon (D-BFT) composite scaffold. The D-BFT scaffold is developed using a combination of physical, chemical, and enzymatic treatments using liquid nitrogen, Triton-X 100, sodium-dodecyl sulfate, and DNase I, which effectively removes the cell components while preserving the biological composite and microstructure. Moreover, the mechanical properties of D-BFT are highly preserved and similar to those of the human Achilles tendon. Additionally, in vitro, mesenchymal stem cells (MSCs) adhered, proliferated, and infiltrated into the D-BFT scaffold, and MSC differentiation is confirmed by up-regulation of osteogenic-related and tenogenic-related genes. The repair outcomes are explored by applying the D-BFT scaffold in the model of femur-tibia defects in vivo, which shows good repair results. Thus, the D-BFT scaffold developed in this study is a promising graft for enthesis regeneration.
肌腱与骨（附着处）断裂，可能导致残疾和持续疼痛，在手术后修复后再次断裂的发生率很高。肌腱或附着处支架已经得到了广泛的研究，但这些材料很少能重现组织连续性。因此，本研究旨在制备三相去细胞骨纤维软骨肌腱（D-BFT）复合支架。D-BFT 支架采用液氮、Triton-X100、十二烷基硫酸钠和 DNase I 的物理、化学和酶处理联合制备，有效地去除细胞成分，同时保留生物复合和微观结构。此外，D-BFT 的机械性能得到高度保留，与人类跟腱相似。此外，在体外，间充质干细胞（MSCs）黏附、增殖并渗透到 D-BFT 支架中，并通过上调成骨相关和肌腱相关基因来证实 MSC 分化。通过将 D-BFT 支架应用于体内股骨-胫骨缺损模型来探索修复效果，结果显示出良好的修复效果。因此，本研究中制备的 D-BFT 支架是一种有前途的附着处再生移植物。
