Donnelly Centre, University of Toronto, Toronto, ON, Canada.
Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Mol Syst Biol. 2019 Aug;15(8):e8679. doi: 10.15252/msb.20188679.
A recent study by Haapaniemi et al (2018) reported that intact p53 signaling hampers CRISPR-based functional genomic screens. Brown et al report good performance of genome-scale screens in TP53 wild-type cells and reiterate best practices for CRISPR screening.
最近，Haapaniemi 等人的一项研究报告称，完整的 p53 信号会阻碍基于 CRISPR 的功能基因组筛选。Brown 等人报告了在 TP53 野生型细胞中进行全基因组筛选的良好性能，并再次强调了 CRISPR 筛选的最佳实践。
