Markowitz M M, Rozen P, Pero R W, Tobi M, Miller D G
Preventive Medicine Institute-Strang Clinic, New York, NY.
Gut. 1988 Dec;29(12):1680-6. doi: 10.1136/gut.29.12.1680.
The sample population in this initial case control study of the adenosine diphosphate ribosyl transferase (ADPRT) response of inflammatory bowel disease patients included: 23 patients with ulcerative colitis (UC)-active and inactive, 13 patients with Crohn's disease (CD)-active and inactive, 14 first degree relatives of UC and CD patients, and 19 age-matched controls. Adenosine diphosphate ribosyl transferase activity was determined after one hour incubation with 1% plasma (the constitutive value) or with 1% plasma and 100 microM H2O2 (the activated value) with the resulting difference designated as the induced value. Statistically significant decrease in ADPRT activity was found for the constitutive, activated and induced values in human mononuclear leucocytes of UC and CD patients, compared with controls. The values in the first degree relatives of UC and CD patients were not significantly different from either the control or disease populations, indicating an intermediate ADPRT response. These results may be related to the nature of the immunological response of IBD patients and comparable with similar findings in other diseases with known DNA repair deficiencies--for example, colon cancer.
在这项关于炎症性肠病患者二磷酸腺苷核糖基转移酶（ADPRT）反应的初始病例对照研究中，样本群体包括：23例溃疡性结肠炎（UC）患者——活动期和非活动期各半，13例克罗恩病（CD）患者——活动期和非活动期各半，14例UC和CD患者的一级亲属，以及19例年龄匹配的对照者。在与1%血浆（本底值）或1%血浆加100微摩尔过氧化氢（激活值）孵育1小时后测定二磷酸腺苷核糖基转移酶活性，所得差值指定为诱导值。与对照者相比，发现UC和CD患者人单核白细胞的本底值、激活值和诱导值的ADPRT活性均有统计学意义的下降。UC和CD患者一级亲属的值与对照人群或疾病人群均无显著差异，表明ADPRT反应处于中间水平。这些结果可能与IBD患者免疫反应的性质有关，并且与其他已知存在DNA修复缺陷的疾病（如结肠癌）的类似发现相似。
