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农杆菌 T-DNAs 的水平基因转移到薯蓣属（Ipomoea 属）基因组中，并不局限于六倍体甘薯。

The horizontal gene transfer of Agrobacterium T-DNAs into the series Batatas (Genus Ipomoea) genome is not confined to hexaploid sweetpotato.

机构信息

Department of Biotechnology, Ghent University, 9000, Ghent, Belgium.

International Potato Center (CIP), Lima, Peru.

出版信息

Sci Rep. 2019 Aug 29;9(1):12584. doi: 10.1038/s41598-019-48691-3.

DOI:10.1038/s41598-019-48691-3
PMID:31467320
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6715720/
Abstract

The discovery of the insertion of IbT-DNA1 and IbT-DNA2 into the cultivated (hexaploid) sweetpotato [Ipomoea batatas (L.) Lam.] genome constitutes a clear example of an ancient event of Horizontal Gene Transfer (HGT). However, it remains unknown whether the acquisition of both IbT-DNAs by the cultivated sweetpotato occurred before or after its speciation. Therefore, this study aims to evaluate the presence of IbT-DNAs in the genomes of sweetpotato's wild relatives belonging to the taxonomic group series Batatas. Both IbT-DNA1 and IbT-DNA2 were found in tetraploid I. batatas (L.) Lam. and had highly similar sequences and at the same locus to those found in the cultivated sweetpotato. Moreover, IbT-DNA1 was also found in I. cordatotriloba and I. tenuissima while IbT-DNA2 was detected in I. trifida. This demonstrates that genome integrated IbT-DNAs are not restricted to the cultivated sweetpotato but are also present in tetraploid I. batatas and other related species.

摘要

IbT-DNA1 和 IbT-DNA2 插入栽培（六倍体）番薯 [Ipomoea batatas (L.) Lam.] 基因组的发现构成了水平基因转移（HGT）的一个古老事件的明确例证。然而，尚不清楚栽培番薯同时获得这两个 IbT-DNAs 是在其物种形成之前还是之后。因此，本研究旨在评估 IbT-DNAs 在属于 Batatas 分类群系列的番薯野生近缘种基因组中的存在。在四倍体 I. batatas (L.) Lam. 中发现了 IbT-DNA1 和 IbT-DNA2，它们的序列高度相似，位于与在栽培番薯中发现的相同位置。此外，在 I. cordatotriloba 和 I. tenuissima 中发现了 IbT-DNA1，而在 I. trifida 中检测到了 IbT-DNA2。这表明整合到基因组中的 IbT-DNAs 不仅局限于栽培番薯，也存在于四倍体 I. batatas 和其他相关物种中。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bd3/6715720/368f56d272ae/41598_2019_48691_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bd3/6715720/5dff3c051f7f/41598_2019_48691_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bd3/6715720/b7674fba6947/41598_2019_48691_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bd3/6715720/97403ee5a41c/41598_2019_48691_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bd3/6715720/3161e7104a29/41598_2019_48691_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bd3/6715720/be4900661739/41598_2019_48691_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bd3/6715720/e3ef98ae1af2/41598_2019_48691_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bd3/6715720/6d28432794fd/41598_2019_48691_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bd3/6715720/03a8643f0e12/41598_2019_48691_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bd3/6715720/2a58d7c27002/41598_2019_48691_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bd3/6715720/3733fa9fbe03/41598_2019_48691_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bd3/6715720/368f56d272ae/41598_2019_48691_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bd3/6715720/5dff3c051f7f/41598_2019_48691_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bd3/6715720/b7674fba6947/41598_2019_48691_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bd3/6715720/97403ee5a41c/41598_2019_48691_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bd3/6715720/3161e7104a29/41598_2019_48691_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bd3/6715720/be4900661739/41598_2019_48691_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bd3/6715720/e3ef98ae1af2/41598_2019_48691_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bd3/6715720/6d28432794fd/41598_2019_48691_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bd3/6715720/03a8643f0e12/41598_2019_48691_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bd3/6715720/2a58d7c27002/41598_2019_48691_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bd3/6715720/3733fa9fbe03/41598_2019_48691_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bd3/6715720/368f56d272ae/41598_2019_48691_Fig11_HTML.jpg

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