Jayabalan Gopal Swamy, Bille Josef F, Mao Xiao Wen, Gimbel Howard V, Rauser Michael E, Wenz Frederik, Fan Joseph T
Clinic for Radiotherapy and Radiation Oncology, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany.
Loma Linda University Eye Institute, Loma Linda, CA 92354, USA.
Biomed Opt Express. 2019 Jun 11;10(7):3217-3231. doi: 10.1364/BOE.10.003217. eCollection 2019 Jul 1.
Safe use of retinal imaging with two-photon excitation in human eyes is crucial, as the effects of ultrashort pulsed lasers on the retina are relatively unknown. At the time of the study, the laser safety standards were inadequate due to the lack of biological data. This article addresses the feasibility of two-photon retinal imaging with respect to laser safety. In this study, rat retinas were evaluated at various laser exposure levels and with different laser parameters to determine the effects of laser-induced optical damage. The results were experimentally verified using confocal reflectance imaging, two-photon fluorescein angiography, immunohistochemistry, and correlated to the IEC 60825-1 laser safety standard.
在人眼中安全使用双光子激发视网膜成像至关重要，因为超短脉冲激光对视网膜的影响相对未知。在该研究开展时，由于缺乏生物学数据，激光安全标准并不完善。本文探讨了双光子视网膜成像在激光安全方面的可行性。在本研究中，对大鼠视网膜在不同激光暴露水平和不同激光参数下进行评估，以确定激光诱导的光学损伤的影响。使用共焦反射成像、双光子荧光血管造影、免疫组织化学对结果进行了实验验证，并与IEC 60825-1激光安全标准相关联。
