Department of Infectious Diseases, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, China.
Department of Clinical Laboratory, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, China.
J Immunol Res. 2019 Aug 6;2019:3783209. doi: 10.1155/2019/3783209. eCollection 2019.
is an intracellular infection bacterium; the pathogenesis of and chronicity of infection may be related to the immune response of T cells. T lymphocytes mainly participate in cellular immune response. The extent of different T cell subsets imbalanced and their function dysregulated in patients with brucellosis remain not explicit. We grouped patients at different stages (acute, chronic, and convalescent). The frequencies of Th1, Th2, Th17, Treg, and PD-1 (programmed cell death protein 1) in peripheral blood were examined by flow cytometry, and the expressions of T lymphocyte cytokines in serum were detected by cytometric bead array. Th1, Th17, and Treg cell immunity was predominant in the acute stage, while Th2, Th17, and Treg cell immunity was predominant in the chronic stage. The expressions of PD-1 on CD4+ and CD8+ T lymphocytes were significantly different in acute and chronic patients. The percentages of Th1 cells in convalescent patients were still higher than those in healthy controls within one year after withdrawal. The expression of T lymphocyte cytokines in serum was different in patients at different stages. These results indicate that peripheral T lymphocyte immunity was involved in patients with brucellosis and represents a target for the preclinical and clinical assessment of novel immunomodulating therapeutics. The patients' immune function had not completely recovered in a short period of time during convalescence, so long-term follow-up of convalescent patients is needed.
是一种胞内感染细菌；其感染的发病机制和慢性化可能与 T 细胞免疫反应有关。T 淋巴细胞主要参与细胞免疫反应。不同 T 细胞亚群失衡及其功能失调在布鲁氏菌病患者中的程度尚不清楚。我们将不同阶段（急性、慢性和恢复期）的患者进行分组。通过流式细胞术检测外周血中 Th1、Th2、Th17、Treg 和 PD-1（程序性细胞死亡蛋白 1）的频率，并通过细胞因子检测试剂盒检测血清中 T 淋巴细胞细胞因子的表达。急性阶段以 Th1、Th17 和 Treg 细胞免疫为主，慢性阶段以 Th2、Th17 和 Treg 细胞免疫为主。急性和慢性患者的 CD4+和 CD8+T 淋巴细胞上 PD-1 的表达差异有统计学意义。在停药 1 年内，恢复期患者的 Th1 细胞百分比仍高于健康对照组。不同阶段患者血清中 T 淋巴细胞细胞因子的表达不同。这些结果表明，外周 T 淋巴细胞免疫参与了布鲁氏菌病患者的发病过程，是评估新型免疫调节治疗药物的临床前和临床的一个靶点。在恢复期，患者的免疫功能在短时间内尚未完全恢复，因此需要对恢复期患者进行长期随访。
