Dipartimento di Medicina Clinica e Molecolare, Ospedale Sant'Andrea, Facoltà di Medicina e Psicologia, Ospedale Sant'Andrea, "Sapienza, " Università di Roma, Italy.
Dipartimento di Medicina Clinica e Molecolare, Ospedale Sant'Andrea, Facoltà di Medicina e Psicologia, Ospedale Sant'Andrea, UOS Aterosclerosi e Dislipidemia, "Sapienza, " Università di Roma, Italy.
J Immunol Res. 2019 Aug 6;2019:9782594. doi: 10.1155/2019/9782594. eCollection 2019.
Monocytes are a heterogeneous cell population distinguished into three subsets with distinctive phenotypic and functional properties: "classical" (CD14++CD16-), "intermediate" (CD14++CD16+), and "nonclassical" (CD14+CD16++). Monocyte subsets play a pivotal role in many inflammatory systemic diseases including atherosclerosis (ATS). Only a low number of studies evaluated monocyte behavior in patients affected by cardiovascular diseases, and data about their role in acute aortic dissection (AAD) are lacking. Thus, the aim of this study was to investigate CD14++CD16-, CD14++CD16+, and CD14+CD16++ cells in patients with Stanford-A AAD and in patients with carotid artery stenosis (CAS). . 20 patients with carotid artery stenosis (CAS group), 17 patients with Stanford-A AAD (AAD group), and 17 subjects with traditional cardiovascular risk factors (RF group) were enrolled. Monocyte subset frequency was determined by flow cytometry. . Classical monocytes were significantly increased in the AAD group versus CAS and RF groups, whereas intermediate monocytes were significantly decreased in the AAD group versus CAS and RF groups. . Results of this study identify in AAD patients a peculiar monocyte array that can partly explain depletion of T CD4+ lymphocyte subpopulations observed in patients affected by AAD.
单核细胞是一种异质性细胞群体，可分为具有独特表型和功能特性的三个亚群：“经典”（CD14++CD16-）、“中间”（CD14++CD16+）和“非经典”（CD14+CD16++）。单核细胞亚群在许多炎症性全身性疾病中发挥着关键作用，包括动脉粥样硬化（ATS）。只有少数研究评估了患有心血管疾病的患者的单核细胞行为，并且缺乏关于其在急性主动脉夹层（AAD）中的作用的数据。因此，本研究旨在研究斯坦福 A 型 AAD 患者和颈动脉狭窄（CAS）患者中的 CD14++CD16-、CD14++CD16+和 CD14+CD16++细胞。共纳入 20 例颈动脉狭窄（CAS 组）、17 例斯坦福 A 型 AAD（AAD 组）和 17 例具有传统心血管危险因素（RF 组）的患者。通过流式细胞术确定单核细胞亚群频率。AAD 组的经典单核细胞显著高于 CAS 组和 RF 组，而中间单核细胞在 AAD 组中显著低于 CAS 组和 RF 组。本研究的结果确定了 AAD 患者中存在一种特殊的单核细胞阵列，它可以部分解释在 AAD 患者中观察到的 T CD4+淋巴细胞亚群耗竭。
