Xiang Yang, Liang Bin, Zhang Xiaokang, Zheng Fang
Center for Gene Diagnosis, and Clinical Lab, Zhongnan Hospital of Wuhan University, Donghu Road 169, Wuhan 430071, China.
Int J Med Sci. 2020 Aug 1;17(14):2171-2179. doi: 10.7150/ijms.47998. eCollection 2020.
Increased expressions of CD16 on classical monocytes precede their transition to intermediate monocytes. Thus far, the influence of lipids on the expression of CD14 and CD16 on monocyte subsets in coronary atherosclerosis (CA) remains unclear. The aim of this study was to investigate the underlying association between blood lipids and the expression of CD14 and CD16 on monocyte subsets. This study enrolled 112 healthy controls and 110 CA patients. Monocyte subsets [CD14CD16 (classical), CD14CD16 (intermediate) and CD14CD16 (non-classical)] were analyzed by flow cytometry. Median fluorescent intensity (MFI) was used to evaluate the expression levels of CD14 and CD16 on monocyte subsets. Compared with the control group, the expression of CD16 was significantly increased on all three monocyte subsets in the patient group. Correlation analysis revealed that serum HDL-C was inversely associated with the expression of CD16 on intermediate monocytes after Bonferroni correction in the control group. In addition, a significant decrease in classical monocytes and an increase in intermediate monocytes were detected in patients. In linear regression analysis, intermediate monocytes showed an inverse association with serum HDL-C in the control group. Although CD14 was correlated with serum TC and HDL-C, there was no statistical difference in CD14 expression between the two groups. Low serum HDL-C may induce upregulation of CD16 on classical monocytes, which may in turn lead to the increase of intermediate monocytes in coronary atherosclerosis patients.
经典单核细胞向中间单核细胞转变之前，其CD16表达增加。迄今为止，脂质对冠状动脉粥样硬化（CA）中单核细胞亚群CD14和CD16表达的影响仍不清楚。本研究旨在探讨血脂与单核细胞亚群CD14和CD16表达之间的潜在关联。本研究纳入了112名健康对照者和110名CA患者。通过流式细胞术分析单核细胞亚群[CD14⁺CD16⁻（经典型）、CD14⁺CD16⁺（中间型）和CD14⁻CD16⁺（非经典型）]。采用中位荧光强度（MFI）评估单核细胞亚群上CD14和CD16的表达水平。与对照组相比，患者组所有三个单核细胞亚群上CD16的表达均显著增加。相关性分析显示，在对照组经Bonferroni校正后，血清高密度脂蛋白胆固醇（HDL-C）与中间单核细胞上CD16的表达呈负相关。此外，在患者中检测到经典单核细胞显著减少，中间单核细胞增加。在线性回归分析中，对照组中间单核细胞与血清HDL-C呈负相关。虽然CD14与血清总胆固醇（TC）和HDL-C相关，但两组之间CD14表达无统计学差异。低血清HDL-C可能诱导经典单核细胞上CD16上调，这可能进而导致冠状动脉粥样硬化患者中间单核细胞增加。
