College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road, Yangling, 712100, Shaanxi, China.
College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, 471023, Henan, China.
Appl Microbiol Biotechnol. 2019 Oct;103(20):8545-8557. doi: 10.1007/s00253-019-10095-8. Epub 2019 Aug 29.
Salmonella Typhimurium, a common Gram-negative foodborne pathogen, threatens public health and hinders the development of the food industry. In this study, we evaluated the antibiofilm activity of coenzyme Q0 (CoQ0) against S. Typhimurium. Besides, the inhibition of the S. Typhimurium's adhesion to and invasion of Caco-2 cells and its survival and replication in RAW 264.7 cells by CoQ0 were also explored. The minimum inhibitory concentrations and minimal bactericidal concentrations of CoQ0 against Salmonella were both 100-400 μg/mL. Salmonella Typhimurium biofilm formation was effectively inhibited by subinhibitory concentrations (SICs) of CoQ0. The CoQ0-affected biofilm morphology was observed with light microscopy and field-emission scanning electron microscopy. CoQ0 at SICs reduced the swimming motility and quorum sensing of S. Typhimurium and repressed the transcription of critical virulence-related genes. CoQ0 at SICs also clearly reduced the adhesion of S. Typhimurium to and its invasion of Caco-2 cells and reduced its survival and replication within RAW 264.7 macrophage cells. These findings suggest that CoQ0 has strong antibiofilm activity and can be used as an anti-infectious agent against Salmonella.
肠炎沙门氏菌是一种常见的革兰氏阴性食源性病原体，威胁着公共健康，阻碍了食品工业的发展。在本研究中，我们评估了辅酶 Q0（CoQ0）对肠炎沙门氏菌的抗生物膜活性。此外，还研究了 CoQ0 对肠炎沙门氏菌黏附和侵袭 Caco-2 细胞的抑制作用，以及其在 RAW 264.7 细胞中的存活和复制能力。CoQ0 对沙门氏菌的最小抑菌浓度和最小杀菌浓度均为 100-400 μg/mL。亚抑菌浓度（SIC）的 CoQ0 可有效抑制肠炎沙门氏菌生物膜的形成。用明场显微镜和场发射扫描电子显微镜观察 CoQ0 对生物膜形态的影响。SIC 下的 CoQ0 降低了肠炎沙门氏菌的泳动性和群体感应，并抑制了关键毒力相关基因的转录。SIC 下的 CoQ0 还明显降低了肠炎沙门氏菌对 Caco-2 细胞的黏附和侵袭，降低了其在 RAW 264.7 巨噬细胞内的存活和复制。这些发现表明 CoQ0 具有很强的抗生物膜活性，可作为一种抗沙门氏菌的抗感染剂。
