Farha Arakkaveettil Kabeer, Sui Zhongquan, Corke Harold
Department of Biotechnology and Food Engineering, Guangdong Technion-Israel Institute of Technology, 241 Daxue Road, Shantou 515063, China.
Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Antibiotics (Basel). 2023 Jan 23;12(2):239. doi: 10.3390/antibiotics12020239.
is an important foodborne pathogen that causes gastroenteritis and systemic infection in humans and livestock. biofilms consist of two major components-amyloid curli and cellulose-which contribute to the prolonged persistence of inside the host. Effective agents for inhibiting the formation of biofilms are urgently needed. We investigated the antibiofilm effect of Raspberry Ketone (RK) and its mechanism of action against Typhimurium 14028 using the Congo red agar method, Calcofluor staining, crystal violet method, pellicle assay, and the TMT-labeled quantitative proteomic approach. RK suppressed the formation of different types of biofilms, including pellicle formation, even at low concentrations (200 µg/mL). Furthermore, at higher concentrations (2 mg/mL), RK exhibited bacteriostatic effects. RK repressed cellulose deposition in biofilm through an unknown mechanism. Swimming and swarming motility analyses demonstrated reduced motility in RK-treated typhimurium. Proteomics analysis revealed that pathways involved in amyloid curli production, bacterial invasion, flagellar motility, arginine biosynthesis, and carbohydrate metabolism, were targeted by RK to facilitate biofilm inhibition. Consistent with the proteomics data, the expressions of and genes were strongly down-regulated in RK-treated typhimurium. These findings clearly demonstrated the biofilm inhibition capability of RK, justifying its further study for its efficacy assessment in clinical and industrial settings.
是一种重要的食源性病原体，可导致人类和牲畜患肠胃炎和全身感染。生物膜由两个主要成分组成——淀粉样卷曲蛋白和纤维素，这有助于在宿主体内长期存活。迫切需要有效的抑制生物膜形成的药物。我们使用刚果红琼脂法、荧光增白剂染色法、结晶紫法、菌膜试验和TMT标记的定量蛋白质组学方法，研究了树莓酮（RK）对鼠伤寒沙门氏菌14028的抗生物膜作用及其作用机制。即使在低浓度（200μg/mL）下，RK也能抑制不同类型的生物膜形成，包括菌膜形成。此外，在较高浓度（2mg/mL）下，RK表现出抑菌作用。RK通过未知机制抑制生物膜中纤维素的沉积。游动和群集运动分析表明，经RK处理的鼠伤寒沙门氏菌运动性降低。蛋白质组学分析显示，淀粉样卷曲蛋白产生、细菌侵袭、鞭毛运动、精氨酸生物合成和碳水化合物代谢相关途径是RK作用的靶点，以促进生物膜抑制。与蛋白质组学数据一致，在经RK处理的鼠伤寒沙门氏菌中，和基因的表达强烈下调。这些发现清楚地证明了RK对生物膜的抑制能力，证明了对其在临床和工业环境中的疗效评估进行进一步研究的合理性。
