Markus B H, Colson Y L, Fung J J, Zeevi A, Duquesnoy R J
Department of Pathology, University of Pittsburgh, School of Medicine, Pennsylvania.
Tissue Antigens. 1988 Nov;32(5):241-53. doi: 10.1111/j.1399-0039.1988.tb01663.x.
Flow cytometric analysis and cellular assays were used to determine constitutive and induced expression of class I HLA antigens and class II antigens encoded by the HLA-DR, -DQ and -DP subregions on cultured human arterial endothelial cells (HAEC) derived from transplant donors. Class I but no or minimal quantities of class II HLA antigens were found on HAEC. Prior incubation of HAEC with gamma-IFN increased class I HLA antigen expression and induced class II HLA antigen expression on HAEC. The induced expression of HLA-DQ was lower than that of HLA-DR, but both were significantly reduced in comparison to the frequency of these antigens on EBV transformed lymphoblastoid cell lines derived from the same donor. In addition, supernatants from class I and class II alloreactive clones were shown to induce class II antigen expression on HAEC. By PLT analysis, it was shown that these antigens are functionally capable of generating a lymphocyte response. In this regard, HAEC have proved to be a helpful tool in designing in vitro lymphocyte-endothelial cell studies.
采用流式细胞术分析和细胞试验，以确定来自移植供体的培养人动脉内皮细胞（HAEC）上由HLA - DR、- DQ和 - DP亚区编码的I类HLA抗原和II类抗原的组成型和诱导型表达。在HAEC上发现了I类HLA抗原，但未发现或仅发现少量II类HLA抗原。HAEC预先用γ - 干扰素孵育可增加I类HLA抗原表达，并诱导HAEC上II类HLA抗原表达。HLA - DQ的诱导表达低于HLA - DR，但与来自同一供体的EBV转化淋巴母细胞系上这些抗原的频率相比，两者均显著降低。此外，I类和II类同种异体反应性克隆的上清液显示可诱导HAEC上II类抗原表达。通过血小板淋巴细胞检测（PLT分析）表明，这些抗原在功能上能够产生淋巴细胞反应。在这方面，HAEC已被证明是设计体外淋巴细胞 - 内皮细胞研究的有用工具。
