Li J, Farthing P M, Thornhill M H
Clinical Academic Group of Oral Medicine and Dental Diagnostic Science, University Dental Hospital of Manchester, UK.
Arch Oral Biol. 1996 Jun;41(6):533-8. doi: 10.1016/0003-9969(96)00026-x.
The expression, and cytokine modulation, of major histocompatibility complex (MHC) class I and class II molecules on oral and skin keratinocytes were compared in cell culture. Both cell types expressed class I, but not class II, constitutively. However, stimulation with interferon-gamma, but not interleukin-1 alpha, and -1 beta, tumour necrosis factor-alpha or lymphotoxin, induced increased expression of class I and de-novo expression of HLA-DR on both cell types. Oral keratinocytes differed from skin keratinocytes in that they exhibited greater sensitivity to interferon-gamma stimulation and higher stimulated expression of both class I and HLA-DR. In addition, interferon-gamma stimulated oral, but not skin, keratinocytes to express HLA-DP and -DQ. These observations suggest that, like skin keratinocytes, under certain conditions, oral keratinocytes may be able to act as antigen-presenting cells. This may be important in the initiation and progression of some immune-mediated mucocutaneous diseases. Moreover, differences in MHC expression may help to explain differences in the presentation of these diseases on the skin and oral mucosa.
在细胞培养中比较了口腔和皮肤角质形成细胞上主要组织相容性复合体（MHC）I类和II类分子的表达及细胞因子调节情况。两种细胞类型均组成性表达I类分子，但不表达II类分子。然而，用γ干扰素刺激，而非白细胞介素-1α、-1β、肿瘤坏死因子-α或淋巴毒素刺激，可诱导两种细胞类型的I类分子表达增加以及HLA-DR的从头表达。口腔角质形成细胞与皮肤角质形成细胞的不同之处在于，它们对γ干扰素刺激表现出更高的敏感性，且I类分子和HLA-DR的刺激后表达更高。此外，γ干扰素刺激口腔角质形成细胞表达HLA-DP和-DQ，但不刺激皮肤角质形成细胞。这些观察结果表明，与皮肤角质形成细胞一样，在某些条件下，口腔角质形成细胞可能能够充当抗原呈递细胞。这在某些免疫介导的黏膜皮肤疾病的起始和进展中可能很重要。此外，MHC表达的差异可能有助于解释这些疾病在皮肤和口腔黏膜上表现的差异。
