Freshwater Biosources Utilization Bureau, Bioresources Industrialization Support Division, Nakdonggang National Institute of Biological Resources (NNIBR), Sangju, Republic of Korea.
Department of Food Technology and Nutrition, Chonnam National University, Yeosu, Republic of Korea.
Adv Exp Med Biol. 2019;1155:597-610. doi: 10.1007/978-981-13-8023-5_54.
Octopus ocellatus meat (OM) is well known as a plentiful protein source. In this study, we evaluated the hepatoprotective effect of an aqueous extract of OM (OMA) against HO-triggered oxidative stress in human hepatocytes. First of all, taurine rich OMA showed a good ORAC value and reducing power and it was similar with that of ascorbic acid, which is known as a strong antioxidant. Also, OMA significantly improved HO-decreased cell viability by reducing the generation of intracellular reactive oxygen species (ROS) in hepatocytes. Interestingly, the stimulation of HO-induced the formations of apoptotic bodies and sub-G DNA content, whereas they were inhibited by the treatment with OMA. Furthermore, OMA regulated the protein expression levels of apoptotic molecules, such as Bax and Bcl-2. Taken together, this study suggests that OMA, which contains an abundant amount of taurine, protects hepatocytes from HO-triggered oxidative stress and might be a functional food material with hepatoprotective effects.
眼斑拟石首鱼肌肉（OM）是一种众所周知的富含蛋白质的食物来源。在本研究中，我们评估了眼斑拟石首鱼肌肉水提物（OMA）对 HO 诱导的人肝细胞氧化应激的肝保护作用。首先，富含牛磺酸的 OMA 表现出良好的 ORAC 值和还原能力，与作为强抗氧化剂的抗坏血酸相似。此外，OMA 通过减少细胞内活性氧（ROS）的生成，显著改善了 HO 降低的细胞活力。有趣的是，HO 的刺激诱导了凋亡小体的形成和亚 G1 DNA 含量，而 OMA 的处理抑制了它们的形成。此外，OMA 调节了凋亡分子如 Bax 和 Bcl-2 的蛋白表达水平。总之，这项研究表明，含有丰富牛磺酸的 OMA 可保护肝细胞免受 HO 诱导的氧化应激，可能是一种具有肝保护作用的功能性食品材料。
