Department of Marine Bioindustry, Hanseo University, Seosan, Chungcheongman-do, Republic of Korea.
Department of Food Technology and Nutrition, Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu, Republic of Korea.
Adv Exp Med Biol. 2019;1155:471-482. doi: 10.1007/978-981-13-8023-5_44.
Endothelial cell dysfunction (ECD) is a broad term, which implies dysregulation of endothelial cell functions. Several factors contribute to ECD including high blood pressure, high cholesterol levels, diabetes, obesity, hyperglycemia, and advanced glycation end products (AGEs). The highly reactive dicarbonyl methylglyoxal (MGO) is mainly formed as byproduct of glycolysis. Therefore, high blood glucose levels result in increased MGO accumulation. Taurine-rich foods are considered to protect against various diseases including vasculopathy and to exert anti-aging effects. Here, we investigated the protective effect of hot water extract of Octopus ocellatus meat (OOM), which contains high amounts of taurine, on MGO-induced cell damage in human umbilical vein endothelial cells and zebrafish embryos. Hot water extract of OOMinhibited MGO-induced cytotoxicity and DNA damage, as well as AGEs accumulation. In addition, hot water extract of OOM protected against vascular damage in zebrafish embryos. These results suggest that hot water extract of OOM possesses protective activity against MGO-induced cytotoxicity in both umbilical vein endothelial cells and zebrafish embryos. Therefore, it could be used as a dietary source of an agent for the prevention of vascular diseases.
内皮细胞功能障碍 (ECD) 是一个广义的术语，意味着内皮细胞功能的失调。几个因素促成了 ECD，包括高血压、高胆固醇水平、糖尿病、肥胖、高血糖和晚期糖基化终产物 (AGEs)。高度反应性二羰基甲基乙二醛 (MGO) 主要是糖酵解的副产物形成的。因此，高血糖水平会导致 MGO 积累增加。富含牛磺酸的食物被认为可以预防包括血管病变在内的各种疾病，并具有抗衰老作用。在这里，我们研究了含有大量牛磺酸的章鱼眼肉热水提取物 (OOM) 对人脐静脉内皮细胞和斑马鱼胚胎中 MGO 诱导的细胞损伤的保护作用。OOM 的热水提取物抑制了 MGO 诱导的细胞毒性和 DNA 损伤以及 AGEs 积累。此外，OOM 的热水提取物可防止斑马鱼胚胎的血管损伤。这些结果表明，OOM 的热水提取物具有对抗 MGO 诱导的人脐静脉内皮细胞和斑马鱼胚胎细胞毒性的保护活性。因此，它可以用作预防血管疾病的药物的膳食来源。
