Department of Marine Life Science, Jeju National University, Jeju, Republic of Korea.
Jeju International Marine Science Center for Research and Education, Korea Institute of Ocean Science and Technology (KIOST), Jeju, Republic of Korea.
Adv Exp Med Biol. 2017;975 Pt 2:955-971. doi: 10.1007/978-94-024-1079-2_76.
Gamma ray irradiation causes immune suppressive responses by inducing oxidative stress such as reduction of cell viability and damages in immune cells. In this present study, we investigated whether Octopus ocellatus meet (OM) consisted of a plentiful taurine has protective effects against damages caused by oxidative stress in murine splenocytes. First of all, we prepared the aqueous extract from OM (OMA) and identified it contained a plentiful taurine content. The result also showed that OMA exhibited the antioxidant activity by scavenging DPPH and ABTS radicals and hydrogen peroxide. In addition, OMA improved the cell viability without cytotoxicity in gamma ray-irradiated murine splenocytes. Moreover, OMA significantly reduced the production of reactive oxygen species (ROS) in gamma ray-irradiated splenocytes. In further study, we identified that OMA protected zebrafish embryo via improving the reduced survival rate and decreasing the formation of deformity caused by the exposure of gamma ray irradiation. Also, OMA decreased the production of NO and ROS in gamma ray-irradiated zebrafish embryos as well as the induction of cell death. In these results, this study suggests that the consumption of taurine-rich foods, such as O. ocellatus, may be useful for the useful material for the protection against oxidative stress.
伽马射线照射会通过诱导氧化应激（如细胞活力降低和免疫细胞损伤）引起免疫抑制反应。在本研究中，我们研究了是否富含牛磺酸的章鱼（OM）具有抵抗氧化应激对鼠脾细胞损伤的保护作用。首先，我们从 OM 中制备了水提取物（OMA），并鉴定出它含有丰富的牛磺酸。结果还表明，OMA 通过清除 DPPH 和 ABTS 自由基以及过氧化氢具有抗氧化活性。此外，OMA 在不产生细胞毒性的情况下提高了伽马射线照射的鼠脾细胞活力。此外，OMA 显著降低了伽马射线照射脾细胞中活性氧物质（ROS）的产生。在进一步的研究中，我们确定 OMA 通过提高存活率和降低由伽马射线照射引起的畸形形成来保护斑马鱼胚胎。此外，OMA 还减少了伽马射线照射的斑马鱼胚胎中 NO 和 ROS 的产生以及细胞死亡的诱导。在这些结果中，本研究表明，消耗富含牛磺酸的食物，如章鱼，可能是对抗氧化应激的有用物质。
