蝙蝠蛾拟青霉水提物通过 LPS 诱导的 RAW264.7 细胞中 NF-кB 和 MAPK 信号通路的抗炎作用。
Anti-inflammatory Effects of Batillaria multiformis Water Extracts via NF-кB and MAPK Signaling Pathways in LPS-Induced RAW 264.7 Cells.
机构信息
Department of Applied Life Science, Konkuk University, Chungju, Republic of Korea.
BK21plus Glocal Education Program of Nutraceuticals Development, Konkuk University, Chungju, Republic of Korea.
出版信息
Adv Exp Med Biol. 2019;1155:1001-1014. doi: 10.1007/978-981-13-8023-5_83.
Batillaria multiformis (B. multiformis) belong to gastropods. They live generally in the sandpit of the lagoons and the estuaries of the intertidal zone. Most of them are distributed in Korea, Japan and China. In this study, we investigated the anti-inflammatory potential of B. multiformis water extracts (BMW). The results showed that the extracts significantly decreased the production of nitric oxide (NO) and pro-inflammatory cytokines, including tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in LPS-induced RAW 264.7 macrophages. In addition, the extracts suppressed the protein levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in a dose dependent manner. Further investigation indicated that BMW suppressed phosphorylated c-Jun N-terminal kinase (JNK), extracellular regulated protein kinase (ERK) and p38 through the MAPK signaling pathway and influenced the NF-κB signaling pathway by suppressing the IκBα degradation in LPS-induced RAW 264.7 macrophages.
多形巴蜗牛（B. multiformis）属于腹足纲动物。它们通常生活在泻湖的沙坑和潮间带的河口。它们大多分布在韩国、日本和中国。在这项研究中，我们研究了多形巴蜗牛水提取物（BMW）的抗炎潜力。结果表明，提取物显著降低了 LPS 诱导的 RAW 264.7 巨噬细胞中一氧化氮（NO）和促炎细胞因子，包括肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的产生。此外，提取物以剂量依赖的方式抑制诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2）的蛋白水平。进一步的研究表明，BMW 通过抑制 LPS 诱导的 RAW 264.7 巨噬细胞中 IκBα 的降解，通过抑制 MAPK 信号通路中磷酸化 c-Jun N-末端激酶（JNK）、细胞外调节蛋白激酶（ERK）和 p38 以及影响 NF-κB 信号通路来抑制 MAPK 信号通路。
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