海草作为一种潜在的营养保健品，可降低促炎标志物。
Seagrass as a potential nutraceutical to decrease pro-inflammatory markers.
机构信息
Department of Applied Microbiology & Biochemistry, Sri Padmavati Mahila Visvavidyalayam (Women's University, Tirupati, 517 502, A.P, India.
出版信息
BMC Complement Med Ther. 2024 Jul 10;24(1):260. doi: 10.1186/s12906-024-04532-z.
BACKGROUND
The Pro-inflammatory mediators such as prostaglandin E2, nitric oxide and TNF-α are the key players in the stimulation of the inflammatory responses. Thus, the pro-inflammatory mediators are considered to be potential targets for screening nutraceutical with anti-inflammatory activity.
METHODS
In this context, we explored the anti-inflammatory potency of seagrass extract with western blot (Bio-Rad) analysis by using LPS induced RAW macrophages as in-vitro models, western blot analysis, In-silico methods using Mastero 13.0 software.
RESULTS
The anti-inflammatory activity of Seagrass was demonstrated through down regulation of Pro-inflammatory markers such as Cyclooxygenase-2, induced Nitric oxide synthase and prostaglandin E synthase-1. The results were validated by docking the phytochemical constituents of seagrass namely Isocoumarin, Hexadecanoic acid, and Cis-9 Octadecenoic acid, 1,2 Benzene dicarboxylic acid and beta-sitosterol with TNF-alpha, COX-2, iNOS and PGES-1.
CONCLUSION
The methanolic extract of seagrass Halophila beccarii is a potential nutraceutical agent for combating against inflammation with a significant anti-inflammatory activity.
背景
促炎介质，如前列腺素 E2、一氧化氮和 TNF-α，是刺激炎症反应的关键因素。因此，促炎介质被认为是筛选具有抗炎活性的营养保健品的潜在靶点。
方法
在这种情况下，我们使用 LPS 诱导的 RAW 巨噬细胞作为体外模型，通过 Western blot（Bio-Rad）分析，探索了海草提取物的抗炎效力，使用 Mastero 13.0 软件进行了 Western blot 分析、基于计算机的虚拟筛选。
结果
海草的抗炎活性通过下调促炎标志物来证明，例如环加氧酶-2、诱导型一氧化氮合酶和前列腺素 E 合酶-1。通过将海草的植物化学成分，如异香豆素、十六烷酸、顺-9 十八碳烯酸、1,2-苯二甲酸和β-谷甾醇与 TNF-α、COX-2、iNOS 和 PGES-1 对接，验证了这些结果。
结论
海草 Halophila beccarii 的甲醇提取物是一种有潜力的营养保健品，具有显著的抗炎活性，可用于对抗炎症。
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