Gokul Megha, Arun Kumar Nayanatara, Durgadas Kini Rekha, Blossom Vandana, Kodavanji Bhagyalakshmi, Noojibail Anupama, Murali Nirupama, Vishwanath Rai Santosh Phajir
Kasturba Medical College, Mangalore, Department of Physiology, Manipal Academy of Higher Education, Manipal, Karnataka, India.
Kasturba Medical College, Mangalore, Department of Anatomy, Manipal Academy of Higher Education, Manipal, Karnataka Mangalore, India.
J Basic Clin Physiol Pharmacol. 2019 Aug 30;30(5):jbcpp-2018-0215. doi: 10.1515/jbcpp-2018-0215.
Background In recent years, increased stress in human life has a dual effect on brain and body physiology. Chronic stress takes a toll on physiology as well as on quality of life, ultimately leading to affective disorders. Rodent models are indispensable tools for studying the etiology and progress of depression. C-reactive protein has been proposed as a novel inflammatory marker. Methods Rats were divided into control and experimental stress groups (n = 6 each). The experimental group consisted of rats that were exposed to a set of chronic unpredictable stressors for 15 days. At the end of the 15th day, the animals were anesthetized, and blood samples were collected through cardiac puncture. Then the blood samples were analyzed for selected biochemical and oxidative stress parameters. Results Serum glutamic oxaloacetic transaminase (p < 0.0001), serum glutamic pyruvic transaminase (p < 0.001), serum malondialdehyde (p < 0.0001), total antioxidant level (p < 0.0001), and serum cortisol (p < 0.0001) were significantly increased in the stressed group when compared with the control group. C-reactive protein significantly (p < 0.0001) increased in the stressed group when compared with the control group. Conclusion Our results demonstrate that chronic unpredictable stress ameliorated depression-like behavior, which might have caused the dysregulation of the hypothalamic-pituitary-adrenal axis, causing the imbalance in the biochemical and oxidative parameters increasing the inflammatory markers. The inflammation-induced model of the chronic unpredictable stress model of comorbid depression might provide a variety of new targets for antidepressant therapies.
背景 近年来，人类生活压力的增加对大脑和身体生理产生了双重影响。慢性应激对生理以及生活质量都造成损害，最终导致情感障碍。啮齿动物模型是研究抑郁症病因和进展不可或缺的工具。C反应蛋白已被提出作为一种新型炎症标志物。方法 将大鼠分为对照组和实验应激组（每组n = 6）。实验组由暴露于一组慢性不可预测应激源15天的大鼠组成。在第15天结束时，将动物麻醉，通过心脏穿刺采集血样。然后对血样进行选定的生化和氧化应激参数分析。结果 与对照组相比，应激组血清谷氨酸草酰乙酸转氨酶（p < 0.0001）、血清谷氨酸丙酮酸转氨酶（p < 0.001）、血清丙二醛（p < 0.0001）、总抗氧化水平（p < 0.0001）和血清皮质醇（p < 0.0001）显著升高。与对照组相比，应激组C反应蛋白显著升高（p < 0.0001）。结论 我们的结果表明，慢性不可预测应激改善了抑郁样行为，这可能导致下丘脑 - 垂体 - 肾上腺轴失调，引起生化和氧化参数失衡，增加炎症标志物。慢性不可预测应激合并抑郁症模型的炎症诱导模型可能为抗抑郁治疗提供多种新靶点。
