Park K
Purdue University, School of Pharmacy, West Lafayette, IN 47907.
Biomaterials. 1988 Sep;9(5):435-41. doi: 10.1016/0142-9612(88)90009-9.
A method was developed for synthesizing enzyme-digestible swelling hydrogels. Albumin molecules were modified using glycidyl acrylate to introduce vinyl groups. The functionalized albumin molecules participated as cross-linkers in the polymerization of vinyl monomers, such as acrylic acid or acrylamide. The extent of chemical modification of albumin was an important variable in controlling the cross-linking ability. The albumin in the synthesized hydrogels retained its property of enzymatic digestion by proteolytic enzymes. The kinetics of swelling and enzymatic digestion of the hydrogels were examined using various enzyme concentrations. It was observed that the digestion kinetics were largely determined by the relative concentrations of albumin and enzyme. The potential application of the enzyme-digestible swelling hydrogels as platforms for long-term oral drug delivery is discussed.
开发了一种合成可酶解溶胀水凝胶的方法。使用丙烯酸缩水甘油酯对白蛋白分子进行修饰以引入乙烯基。功能化的白蛋白分子作为交联剂参与乙烯基单体（如丙烯酸或丙烯酰胺）的聚合反应。白蛋白的化学修饰程度是控制交联能力的一个重要变量。合成水凝胶中的白蛋白保留了其被蛋白水解酶酶解的特性。使用不同的酶浓度研究了水凝胶的溶胀和酶解动力学。观察到酶解动力学在很大程度上由白蛋白和酶的相对浓度决定。讨论了可酶解溶胀水凝胶作为长期口服给药平台的潜在应用。
