College of Resources and Environment, Northeast Agricultural University, Harbin, 150030, China.
College of Resources and Environment, Northeast Agricultural University, Harbin, 150030, China.
Ecotoxicol Environ Saf. 2019 Nov 30;184:109595. doi: 10.1016/j.ecoenv.2019.109595. Epub 2019 Aug 27.
Trichloroethylene (TCE), one of 129 kinds of priority pollutants, is the most common halogenated organic pollutant in the environment. To explore the changes in soil physicochemical properties and biological activities then clarify the effects of these factors on bacterial, fungal and actinomycetes communities in Mollisol under TCE stress is the significance of our research. The results indicated that when TCE concentration was greater than 10 mg kg, soil quality declined and soil decomposition of organic matter and cycling of mineral nutrients were inhibited through an effect on soil microbial biomass. Operational taxonomic units (OTUs) richness of the bacteria in Mollisol was altered by TCE contamination. The S and H indices of bacterial communities in Mollisol decreased when 40 mg kg TCE was applied. Meanwhile, the OTU richness of fungi in Mollisol was altered by TCE contamination. The H indices of the fungal communities in Mollisol were inhibited by higher TCE concentrations (20 and 40 mg kg TCE). TCE altered the content of some bacteria, fungi and actinomycetes involved in soil carbon and nitrogen cycling and metabolism, such as Acidobacteria, Proteobacteria, Planctomycetes, Chytridiomycota, Streptomycetales, Pseudonocardiales, Propionibacteriales and Rhizobiales, and thus influenced nutrient cycling and the process of energy metabolism in Mollisol. In addition, redundancy analysis (RDA) results indicated that physicochemical properties and biological activities under TCE contamination significantly affected soil microbial community composition thus confirming that TCE interfered with the carbon and nitrogen cycling and metabolism of soil microorganisms. The results of this study are of great importance for revealing the effects of TCE stress on the microbiological characteristics of Mollisol, and also provide more useful information for determining the potential ecological risk of organic pollutants in Mollisol.
三氯乙烯（TCE）是 129 种优先污染物之一，是环境中最常见的卤代有机污染物。本研究旨在探讨 TCE 胁迫下黑土理化性质和生物活性的变化，阐明这些因素对细菌、真菌和放线菌群落的影响。结果表明，当 TCE 浓度大于 10mg/kg 时，通过对土壤微生物生物量的影响，土壤质量下降，土壤有机质分解和矿质养分循环受到抑制。TCE 污染改变了黑土中细菌的操作分类单元（OTUs）丰富度。当施加 40mg/kg TCE 时，黑土细菌群落的 S 和 H 指数降低。同时，TCE 污染改变了黑土中真菌的 OTUs 丰富度。较高 TCE 浓度（20 和 40mg/kg TCE）抑制了真菌群落的 H 指数。TCE 改变了一些参与土壤碳氮循环和代谢的细菌、真菌和放线菌的含量，如 Acidobacteria、Proteobacteria、Planctomycetes、Chytridiomycota、Streptomycetales、Pseudonocardiales、Propionibacteriales 和 Rhizobiales，从而影响了黑土的养分循环和能量代谢过程。此外，冗余分析（RDA）结果表明，TCE 污染下的理化性质和生物活性显著影响土壤微生物群落组成，证实 TCE 干扰了土壤微生物的碳氮循环和代谢。本研究结果对于揭示 TCE 胁迫对黑土微生物特征的影响具有重要意义，也为确定黑土中有机污染物的潜在生态风险提供了更有用的信息。
