Key Laboratory of Bio-Resource and Eco-Evironment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, PR China.
Key Laboratory of Bio-Resource and Eco-Evironment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, PR China.
Ecotoxicol Environ Saf. 2018 Dec 30;166:116-122. doi: 10.1016/j.ecoenv.2018.09.076. Epub 2018 Sep 22.
Although the effect of heavy metal on soil microbial diversity was widely studied, the interaction among micro-ecological environment in heavy metal contaminated soil was less known. In this study, we systematically investigated the influence of cadmium (Cd) on soil micro-ecological environment (pH, nutrient content, soil enzyme activities, microbial biomass, bacterial and fungal diversities). Results showed that pH values slightly decreased with the Cd level increase, whereas the nutrient content including of Olsen-P (OSP), Alkeline-N (ALN), Olsen-K (OSK) and organic matter (OM) did not show significant difference in different treatments. In contrast to physicochemical properties, the biochemical qualities were easily influenced by Cd pollutant, resulting in soil microbial numbers and enzyme activities significantly decreased. High-throughput sequencing showed that microbial community composition was significantly affected by heavy metal. For bacteria, Actinobacteria abundance significantly decreased in Cd treated soil, corresponding to Proteobacteria and Firmicutes increased. For fungi, the most dominant phyla member (Ascomycota) was significantly decreased whereas Zygomycota significantly increased with Cd addition. These results further revealed the integral interrelation of micro-ecology environmental players under the stress of different Cd levels.
尽管重金属对土壤微生物多样性的影响已得到广泛研究，但对重金属污染土壤中微生态环境之间的相互作用知之甚少。在本研究中，我们系统地调查了镉 (Cd) 对土壤微生态环境（pH 值、养分含量、土壤酶活性、微生物生物量、细菌和真菌多样性）的影响。结果表明，随着 Cd 水平的升高，pH 值略有下降，而不同处理之间的养分含量（包括 Olsen-P（OSP）、碱性-N（ALN）、Olsen-K（OSK）和有机质（OM））没有显著差异。与理化性质相比，生物化学性质更容易受到 Cd 污染物的影响，导致土壤微生物数量和酶活性显著下降。高通量测序表明，重金属对微生物群落组成有显著影响。对于细菌，Cd 处理土壤中放线菌丰度显著下降，而变形菌门和厚壁菌门丰度增加。对于真菌，最主要的门成员（子囊菌门）显著减少，而接合菌门则随着 Cd 的添加而显著增加。这些结果进一步揭示了在不同 Cd 水平胁迫下微生态环境因子之间的整体相互关系。
