School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Institute of TCM International Standardization of Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Molecules. 2019 Nov 7;24(22):4033. doi: 10.3390/molecules24224033.
As our ongoing research project on Ban Lan Gen ( roots), a total of 23 alkaloids were obtained. Compounds and contain an unusual C-C bond between the 2(1)-quinolinone moiety and the phenol moiety and between the 2(1)-quinolinone moiety and the 1-indole moiety, respectively. Compound possesses an unusual carbon skeleton and its putative biosynthetic pathway was discussed, and Compound was deduced as a new indole alkaloid glycoside. Compounds were identified as four new natural products by extensive spectroscopic experiments. Additionally, the anti-inflammatory activity was assessed based on nitric oxide (NO) production using Lipopolysaccharide-stimulated RAW264.7 macrophages. Compounds , and showed inhibitory effects with IC values of 1.2, 5.0, and 74.4 μM.
作为我们正在进行的板蓝根（根）研究项目，共获得了 23 种生物碱。化合物和含有在 2(1)-喹啉酮部分和酚部分之间以及在 2(1)-喹啉酮部分和 1-吲哚部分之间不寻常的 C-C 键。化合物具有不寻常的碳骨架，讨论了其可能的生物合成途径，化合物被推断为一种新的吲哚生物碱糖苷。通过广泛的光谱实验，将化合物鉴定为四种新的天然产物。此外，基于脂多糖刺激的 RAW264.7 巨噬细胞中一氧化氮（NO）的产生来评估抗炎活性。化合物、和表现出抑制作用，IC 值分别为 1.2、5.0 和 74.4 μM。
