School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, People's Republic of China; School of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang 712046, People's Republic of China.
School of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang 712046, People's Republic of China.
Fitoterapia. 2020 Oct;146:104654. doi: 10.1016/j.fitote.2020.104654. Epub 2020 Jun 2.
Eight bisindole alkaloids including six undescribed ones (1a/1b-5) were isolated from an alcohol extract of the Isatis indigotica roots. Their structures and absolute configurations were supported by extensive spectroscopic data analysis, including 1D, 2D NMR, HRESIMS data, specific rotation data, and comparison of the experimental and calculated ECD data. Compounds 1a and 1b were determined to be a pair of enantiomers with a ratio of approximately 1:1 by chiral-phase chromatography analysis while compound 4 was elucidated as a new type of bisindole alkaloid with the aglycone categorized as bis(indole-1'/3″-yl)methane. All the isolated compounds were tested for their nitric oxide (NO) inhibitory effects and 1-4 and 6 exhibited inhibitory effects with IC values ranging from 11.0 to 37.6 μM.
从菘蓝根的醇提物中分离得到了 8 个双吲哚生物碱，其中包括 6 个未被描述的化合物（1a/1b-5）。它们的结构和绝对构型通过广泛的光谱数据分析得到支持，包括 1D、2D NMR、HRESIMS 数据、比旋光度数据以及实验和计算 ECD 数据的比较。通过手性色谱分析，确定化合物 1a 和 1b 为一对对映异构体，比例约为 1:1，而化合物 4 被阐明为一种新型的双吲哚生物碱，其糖苷配基归类为双（吲哚-1'/3″-基）甲烷。所有分离得到的化合物均进行了一氧化氮（NO）抑制活性测试，结果显示化合物 1-4 和 6 具有抑制活性，IC 值范围为 11.0-37.6 μM。
