Corneli Sara, Corte Laura, Roscini Luca, Di Paolo Antonella, Colabella Claudia, Petrucci Linda, Severi Giulio, Cagiola Monica, Mazzone Piera
Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche "Togo Rosati", Via Gaetano Salvemini 1, 06126 Perugia, Italy.
Department of Pharmaceutical Sciences-Microbiology section, University of Perugia, Borgo 20 Giugno 74, 06121 Perugia, Italy.
Pathogens. 2019 Aug 29;8(3):136. doi: 10.3390/pathogens8030136.
Tuberculins purified protein derivatives (PPDs) are obtained by precipitation from heat treated mycobacteria. PPDs are used in diagnosis of mycobacterial infections in humans and animals. Bovine PPD (PPDB) is obtained from ( complex), while Avian PPD (PPDA) and Johnin PPD (PPDJ) are extracted, respectively, from and ( complex). PPDB and PPDA are used for bovine tuberculosis diagnosis, while PPDJ is experimentally used in the immunodiagnosis of paratuberculosis. Although PPDs date back to the 19th Century, limited knowledge about their composition is currently available. The goal of our study was to evaluate Fourier Transform InfraRed (FTIR) spectroscopy as a tool to differentiate PPDB, PPDA, and three PPDJs. The results highlighted that the three PPDs have specific profiles, correlated with phylogenetic characteristics of mycobacteria used for their production. This analysis is eligible as a specific tool for different PPDs batches characterization and for the assessment of their composition. The entire PPD production may be efficiently controlled, since the N content of each preparation is related to IR spectra, with a reference spectrum for each PPD and a standardized analysis protocol.
结核菌素纯蛋白衍生物（PPD）是通过从热处理的分枝杆菌中沉淀获得的。PPD用于人类和动物分枝杆菌感染的诊断。牛PPD（PPDB）从（复合体）获得，而禽PPD（PPDA）和副结核菌素PPD（PPDJ）分别从（复合体）和（复合体）中提取。PPDB和PPDA用于牛结核病诊断，而PPDJ在实验中用于副结核病的免疫诊断。尽管PPD可追溯到19世纪，但目前关于其组成的知识有限。我们研究的目的是评估傅里叶变换红外（FTIR）光谱作为区分PPDB、PPDA和三种PPDJ的工具。结果表明，这三种PPD具有特定的谱图，与用于生产它们的分枝杆菌的系统发育特征相关。该分析有资格作为不同PPD批次表征及其组成评估的特定工具。由于每种制剂的氮含量与红外光谱相关，每种PPD都有参考光谱和标准化分析方案，因此可以有效地控制整个PPD的生产。
