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一种用于定量分析冰岛衣中松萝酸的可靠液相色谱-串联质谱法的开发与验证。

Development and validation of a reliable LC-MS/MS method for quantitative analysis of usnic acid in Cladonia uncialis.

作者信息

Sveshnikova Natalia, Yuan Tao, Warren Jamie M, Piercey-Normore Michele D

机构信息

School of Science and the Environment, Grenfell Campus, Memorial University of NL, 20 University Drive, Corner Brook, NL, A2H 5G4, USA.

School of Science and the Environment, Boreal Ecosystem Research Facility, Grenfell Campus, Memorial University, 20 University Dr, Corner Brook, NL, A2H 5G4, USA.

出版信息

BMC Res Notes. 2019 Aug 30;12(1):550. doi: 10.1186/s13104-019-4580-x.

DOI:10.1186/s13104-019-4580-x
PMID:31470895
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6716858/
Abstract

OBJECTIVE

The purpose of this study was to develop and validate a specific and sensitive liquid chromatography tandem mass-spectrometry method for quantification of usnic acid concentration in the lichen, Cladonia uncialis, suitable for detection of relatively small fluctuations of usnic acid concentration in response to environmental changes.

RESULTS

The resulting method was fully validated according to international guidelines and demonstrated good selectivity and sensitivity with minor levels of a matrix effect and high accuracy.

摘要

目的

本研究的目的是开发并验证一种特定且灵敏的液相色谱串联质谱法，用于定量测定地衣松萝中松萝酸的浓度，该方法适用于检测松萝酸浓度因环境变化而产生的相对较小的波动。

结果

所得方法根据国际指南进行了充分验证，显示出良好的选择性和灵敏度，基质效应较小且准确性高。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c51/6716858/34a745eae8f0/13104_2019_4580_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c51/6716858/892b87f7c12b/13104_2019_4580_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c51/6716858/34a745eae8f0/13104_2019_4580_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c51/6716858/892b87f7c12b/13104_2019_4580_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c51/6716858/34a745eae8f0/13104_2019_4580_Fig2_HTML.jpg

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本文引用的文献

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Identification of 6-Hydroxymellein Synthase and Accessory Genes in the Lichen Cladonia uncialis.鉴定地卷衣属 Cladonia uncialis 中的 6-羟基杨梅素合酶及其辅助基因。
J Nat Prod. 2016 Jun 24;79(6):1645-50. doi: 10.1021/acs.jnatprod.6b00257. Epub 2016 Jun 6.
2
Effect of aposymbiotic conditions on colony growth and secondary metabolite production in the lichen-forming fungus Ramalina dilacerata.无菌条件对地衣真菌拉玛利纳·迪拉塞塔的菌落生长和次生代谢产物产生的影响。
Fungal Biol. 2013 Nov-Dec;117(11-12):731-43. doi: 10.1016/j.funbio.2013.09.003. Epub 2013 Sep 27.
3
In situ imaging of usnic acid in selected Cladonia spp. by vibrational spectroscopy.
Front Microbiol. 2023 Apr 17;14:1177123. doi: 10.3389/fmicb.2023.1177123. eCollection 2023.
利用振动光谱原位成像法观察选定石松属 Cladonia spp.中的 usnic acid。
Analyst. 2010 Dec;135(12):3242-8. doi: 10.1039/c0an00533a. Epub 2010 Oct 7.
4
Determination of usnic acid in lichen toxic to elk by liquid chromatography with ultraviolet and tandem mass spectrometry detection.采用液相色谱-紫外和串联质谱检测法测定对麋鹿有毒的地衣中的松萝酸。
J Agric Food Chem. 2006 Apr 5;54(7):2484-90. doi: 10.1021/jf052767m.
5
Usnic acid: a non-genotoxic compound with anti-cancer properties.松萝酸：一种具有抗癌特性的非遗传毒性化合物。
Anticancer Drugs. 2005 Sep;16(8):805-9. doi: 10.1097/01.cad.0000175588.09070.77.
6
Seasonal trends in usnic acid concentrations of Arctic, alpine and Patagonian populations of the lichen Flavocetraria nivalis.极地雪茶（Flavocetraria nivalis）在北极、高山和巴塔哥尼亚地区种群中的松萝酸浓度的季节性变化趋势。
Phytochemistry. 2005 Feb;66(3):337-44. doi: 10.1016/j.phytochem.2004.12.007.
7
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Oecologia. 2005 Mar;143(1):94-105. doi: 10.1007/s00442-004-1768-z. Epub 2004 Dec 24.
8
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9
A review on usnic acid, an interesting natural compound.关于扁枝衣酸，一种有趣的天然化合物的综述。
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