Van den Bergh P, Wu P, Jackson I M, Lechan R M
Department of Medicine, New England Medical Center, Boston, MA 02111.
Brain Res. 1988 Sep 27;461(1):53-63. doi: 10.1016/0006-8993(88)90724-x.
Using an antiserum (no. 373) raised against a tyrosinated analog of preproTRH53-74 [( Tyr1]preproTRH53-74 or pYT 22), we have demonstrated the presence of a discrete population of immunoreactive neurons in the midbrain periaqueductal gray (PAG). Relative to the distribution of serotonin, somatostatin, peptide histidine isoleucine (PHI), methionine enkephalin, substance P and neurotensin-containing neuronal perikarya in the PAG, neurons containing immunoreactive pYT 22 occupied a unique location in the ventrolateral PAG. In contrast, terminal fields containing these neuroactive substances with the exception of PHI, were seen in abundance in the region of the ventrolateral PAG neurons. These studies indicate that a non-TRH sequence contained within the N-terminal portion of the TRH prohormone are expressed in a distinct group of neurons in the ventrolateral PAG. The location of these neurons in the PAG in a region richly innervated by nerve terminals containing analgesia-mediating substances, suggests a possible role for proTRH-derived peptides in the modulation of nociception.
利用一种针对前促甲状腺激素释放激素53 - 74的酪氨酸化类似物（[Tyr1]前促甲状腺激素释放激素53 - 74或pYT 22）制备的抗血清（编号373），我们已证实在中脑导水管周围灰质（PAG）中存在一群离散的免疫反应性神经元。相对于PAG中5-羟色胺、生长抑素、肽组氨酸异亮氨酸（PHI）、甲硫氨酸脑啡肽、P物质和含神经降压素的神经元胞体的分布，含免疫反应性pYT 22的神经元在腹外侧PAG中占据独特位置。相比之下，除PHI外，含有这些神经活性物质的终末场所在腹外侧PAG神经元区域大量可见。这些研究表明，促甲状腺激素释放激素原激素N端部分所含的非促甲状腺激素释放激素序列在腹外侧PAG的一组独特神经元中表达。这些神经元在PAG中位于一个由含镇痛介导物质的神经终末丰富支配的区域，提示促甲状腺激素释放激素原衍生肽在伤害感受调制中可能发挥作用。
