Liao N, Bulant M, Nicolas P, Vaudry H, Pelletier G
MRC Group in Molecular Endocrinology, Le Centre Hospitalier de l'Université Laval, Quebec, Canada.
Neurosci Lett. 1988 Feb 15;85(1):24-8. doi: 10.1016/0304-3940(88)90422-3.
Using an antiserum to a 22-amino acid synthetic peptide corresponding to sequence 178-199 of the precursor of thyrotropin-releasing hormone (pro-TRH), we have studied by immunocytochemistry the distribution of immunoreactive pro-TRH in the rat brain. Immunostaining found in several regions of the brain had a distribution similar to that previously reported for TRH. Reaction product was found in both neuronal cell bodies and processes. These results indicate that peptide(s) derived from pro-TRH are transported along the axons with TRH itself. Moreover, the presence of immunoreactive nerve endings in different areas including the median eminence suggests that this material might have a neuromodulator/neurotransmitter role in the central nervous system and could also be released into the pituitary portal plexus.
利用针对促甲状腺激素释放激素前体（pro-TRH）序列178 - 199的22个氨基酸合成肽的抗血清，我们通过免疫细胞化学方法研究了大鼠脑中免疫反应性pro-TRH的分布。在脑的几个区域发现的免疫染色分布与先前报道的促甲状腺激素释放激素（TRH）的分布相似。在神经元细胞体和突起中均发现了反应产物。这些结果表明，源自pro-TRH的肽与TRH自身一起沿轴突运输。此外，在包括正中隆起在内的不同区域存在免疫反应性神经末梢，这表明这种物质可能在中枢神经系统中具有神经调质/神经递质的作用，并且也可能释放到垂体门脉丛中。
