使用无限制体干细胞评估不同膜表面的生物相容性。
Evaluation of Biocompatibility of Different Membrane Surfaces Using Unrestricted Somatic Stem Cells.
机构信息
Department of Oral-, Maxillo- and Plastic Facial Surgery, Heinrich-Heine-University and University Hospital Duesseldorf, Duesseldorf, Germany.
Klinik und Praxisgemeinschaft am Kaiserteich, Düsseldorf, Germany
出版信息
In Vivo. 2019 Sep-Oct;33(5):1447-1454. doi: 10.21873/invivo.11623.
BACKGROUND/AIM: Results of Guided Bone Regeneration (GBR) primarily depend on the membrane used. The aim of this study was to compare biocompatibility of different absorbable and non-absorbable membranes by using unrestricted somatic stem cells (USSCs) as an indicator for biocompatibility.
MATERIALS AND METHODS
Five absorbable membranes (Bio-Gide®, RESODONT®, GENTA-FOIL resorb®, BioMend® and BioMend® Extend™) and one non-absorbable alternative (GORE-TEX®) were colonized with USSCs. After 24 h, 3 days and 7 days, cell proliferation, cell viability, and cytotoxicity were assessed. Moreover, cell morphology was evaluated by electron microscopy.
RESULTS
Significantly higher cell proliferation and cell viability rates were observed in Bio-Gide® and RESODONT® membranes. Cell toxicity was highest on GENTA-FOIL resorb® membranes. The electron microscopical assessment showed a better cell attachment on porous surfaced membranes.
CONCLUSION
This study shows that USSCs can be used for assessments of biocompatibility, and that absorbable membranes with collagenous composition and porous structure tend to positively impact biocompatibility and enhance cell proliferation.
背景/目的：引导骨再生（GBR）的结果主要取决于所使用的膜。本研究旨在通过使用无限制的体干细胞（USSCs）作为生物相容性的指标，比较不同可吸收和不可吸收膜的生物相容性。
材料和方法
用 USSCs 接种 5 种可吸收膜（Bio-Gide®、RESODONT®、GENTA-FOIL resorb®、BioMend®和 BioMend® Extend™）和 1 种不可吸收的膜（GORE-TEX®）。24 小时、3 天和 7 天后，评估细胞增殖、细胞活力和细胞毒性。此外，通过电子显微镜评估细胞形态。
结果
在 Bio-Gide®和 RESODONT®膜中观察到更高的细胞增殖和细胞活力。GENTA-FOIL resorb®膜的细胞毒性最高。电子显微镜评估显示，多孔表面膜上的细胞附着更好。
结论
本研究表明，USSCs 可用于生物相容性评估，具有胶原组成和多孔结构的可吸收膜往往会对生物相容性产生积极影响，并增强细胞增殖。
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