Emami Tahereh, Mirzaeiheydari Mohammad, Maleki Abbas, Bazgir Masoud
Department of Agronomy and Plant Breeding, College of Agriculture, Ilam Branch, Islamic Azad University, Ilam, Iran.
Department of Soil and Water Engineering, Faculty of Agriculture, Ilam University, Ilam, Iran.
Cell Mol Biol (Noisy-le-grand). 2019 Aug 5;65(6):22-27.
Salinity is one of the main obstacles to the production of crops in dry regions of the world. This study focuses on the effects of different strains of plant growth promoting rhizobacteria (PGPR) isolated from native soils on the physiological responses of wheat and barley plants under normal and salt stress conditions. Soil samples were collected from a field in Ilam province, in Iran and bacterial isolates were isolated and screened for salt tolerance, included siderophore and ACC-deaminase production and phosphate solubilizing. Thereafter a two-years greenhouse experiment was conducted as a completely randomized block design with four replications. The applied treatments included bacterial inoculation at five levels (B0: non-inoculation, B1: Siderophore producing + salt-tolerant bacteria, B2: phosphate solubilizing + salt-tolerant bacteria, B3: ACC-deaminase producing + salt-tolerant bacteria, B4: Barvar-2 biological fertilizer, B5: Biofarm-2 biological fertilizer) and salt stress at three levels (S1: 0 dS/m, S2: 4 dS/m, S3: 8 dS/m). Results showed that phosphate solubilizing+ salt-tolerant bacteria resulted in the highest barley grain yield at 4 dS/m salinity level and had no significant difference with ACC-deaminase producing + salt-tolerant bacteria and Barvar-2 biological fertilizer and Biofarm-2 biological fertilizer. The highest proline content in wheat and barley observed in Siderophore producing+ salt-tolerant bacteria at 8 dS/m by 17.48 and 23.42, respectively, followed by phosphate solubilizing+ salt-tolerant bacteria by 16.53 and 19.78. Therefore, the application of isolated growth promoting bacteria can be recommended as an effective biofertilizer in Ilam province.
盐度是世界干旱地区作物生产的主要障碍之一。本研究聚焦于从当地土壤中分离出的不同植物生长促生根际细菌（PGPR）菌株，对正常和盐胁迫条件下小麦和大麦植株生理反应的影响。从伊朗伊拉姆省的一块田地采集土壤样本，分离并筛选细菌分离株的耐盐性，包括铁载体和ACC脱氨酶的产生以及磷的溶解。此后，进行了为期两年的温室试验，采用完全随机区组设计，重复四次。应用的处理包括五个水平的细菌接种（B0：不接种，B1：产生铁载体的耐盐细菌，B2：解磷耐盐细菌，B3：产生ACC脱氨酶的耐盐细菌，B4：Barvar-2生物肥料，B5：Biofarm-2生物肥料）和三个水平的盐胁迫（S1：0 dS/m，S2：4 dS/m，S3：8 dS/m）。结果表明，在盐度为4 dS/m时，解磷耐盐细菌使大麦产量最高，与产生ACC脱氨酶的耐盐细菌、Barvar-2生物肥料和Biofarm-2生物肥料相比无显著差异。在盐度为8 dS/m时，产生铁载体的耐盐细菌使小麦和大麦中的脯氨酸含量最高，分别为17.48和23.42，其次是解磷耐盐细菌，分别为16.53和19.78。因此，在伊拉姆省，推荐应用分离出的促生长细菌作为有效的生物肥料。
