Department of Physics, Simon Fraser University, Burnaby, BC, Canada.
MEMPHYS - International and Interdisciplinary Research Network, Denmark; Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark.
Biochim Biophys Acta Biomembr. 2019 Dec 1;1861(12):183053. doi: 10.1016/j.bbamem.2019.183053. Epub 2019 Aug 28.
Two dimensional phase separation in lipid membranes and cell membranes is of interest to biology because of the idea of membrane rafts - compositionally heterogeneous liquid crystal domains with cellular functions. Few quantitative tools exist for characterizing and differentiating coexisting phases on a molecular scale. Lipid acyl chain order can be measured directly using deuterium nuclear magnetic resonance spectroscopy (H NMR), or inferred using fluorescence microscopy along with the environment-sensitive probe Laurdan. We found a linear relationship between the H NMR order parameter and Laurdan generalized polarization. This observed correlation supports the idea that lipid chain order is tightly associated with the amount and dynamics of water molecules at the glycerol backbone level of the membrane.
脂质双层膜和细胞膜中的二维相分离引起生物学的关注，这是因为有膜筏的概念存在-具有细胞功能的组成异质液晶域。目前几乎没有定量工具可用于在分子尺度上对共存相进行特征描述和区分。可以使用氘核磁共振波谱（H NMR）直接测量脂质酰基链有序性，或者使用荧光显微镜以及环境敏感探针 Laurdan 进行推断。我们发现 H NMR 序参数与 Laurdan 广义极化之间存在线性关系。这种观察到的相关性支持这样一种观点，即脂质链有序性与甘油骨架水平的水分子的数量和动态紧密相关。
