Robbins L G, Swanson E E
Department of Zoology, Michigan State University, East Lansing 48824.
Genetics. 1988 Dec;120(4):1053-9. doi: 10.1093/genetics/120.4.1053.
Rex-induced mitotic recombination was used to determine whether nucleolus organizers can pair in both inverted and noninverted orientations. Two target chromosomes, each duplicated for the rDNA region, were exposed to maternal Rex activity. Recombination in one orientation should yield deletion of the material between the two nucleolus organizers, recombination in the other orientation should yield inversion of the same material. Both products were recovered from both target chromosomes. The generality of using Rex-mediated recombination for analysis of the rDNA is considered.
利用雷克斯诱导的有丝分裂重组来确定核仁组织区在反向和非反向取向中是否都能配对。两条靶染色体，每条都在核糖体DNA区域进行了复制，使其暴露于母本雷克斯活性之下。一个取向的重组应导致两个核仁组织区之间的物质缺失，另一个取向的重组应导致相同物质的倒位。两种产物都从两条靶染色体中回收得到。本文考虑了使用雷克斯介导的重组来分析核糖体DNA的普遍性。
