School of Food and Biological Engineering, Engineering Research Center of Bio-process of Ministry of Education, Hefei University of Technology, Hefei, 230009, PR China.
School of Food and Biological Engineering, Engineering Research Center of Bio-process of Ministry of Education, Hefei University of Technology, Hefei, 230009, PR China; Anhui Province Key Laboratory of Functional Compound Seasoning, Anhui Qiangwang Seasoning Food Co., Ltd., Jieshou, 236500, Anhui, PR China.
Food Chem Toxicol. 2019 Nov;133:110796. doi: 10.1016/j.fct.2019.110796. Epub 2019 Aug 28.
Effects of red raspberry extract (RRE) intake on hyperlipidemia mice induced by high-fat diet (HFD) were investigated in this study. After intragastric gavage of RRE for 8 weeks, the body weight and the adipose tissue mass of mice in RRE administration groups significantly (p < 0.05) decreased compared to the group without RRE treatment. RRE treatment significantly (p < 0.05) lowered triglyceride and total cholesterol levels of hyperlipidemia mice. Pparα, Hmgcr, Ldlr, Cyp7a1, Acsl3, Pnpla2 and Pin4 were confirmed as the regulatory genes by transcriptome analysis and qRCR validation. According to KEGG pathway analysis, target genes such as Cyp7a1 and Pin4 were further regulated by the activation of PPARα resulting from RRE supplementation. Meanwhile, liver cholesterol synthesis and conversion were inhibited by the expressions of Hmgcr and Cyp7a1 genes regulated by RRE intake, and Ldlr gene was down-regulated to limit the transport of cholesterol. In addition, RRE treatment could accelerate the conversion from triglyceride to fatty acid. To conclusion, RRE intake would be a protection against diet-induced hypertriglyceridemia.
本研究旨在探讨红树莓提取物（RRE）对高脂饮食（HFD）诱导的高血脂小鼠的影响。经过 8 周的灌胃 RRE 后，与未接受 RRE 治疗的组相比，RRE 给药组的小鼠体重和脂肪组织质量显著（p<0.05）降低。RRE 治疗可显著（p<0.05）降低高血脂小鼠的甘油三酯和总胆固醇水平。通过转录组分析和 qRCR 验证，证实了 Pparα、Hmgcr、Ldlr、Cyp7a1、Acsl3、Pnpla2 和 Pin4 是调节基因。根据 KEGG 通路分析，RRE 补充后激活 PPARα 进一步调节 Cyp7a1 和 Pin4 等靶基因。同时，RRE 摄入可通过调节 Hmgcr 和 Cyp7a1 基因的表达抑制肝脏胆固醇合成和转化，下调 Ldlr 基因以限制胆固醇的转运。此外，RRE 治疗可加速甘油三酯向脂肪酸的转化。综上所述，RRE 的摄入可预防饮食诱导的高甘油三酯血症。
