Key Laboratory of Forestry Genetics & Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing Forestry University, Nanjing 210037, People's Republic of China; Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People's Republic of China.
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People's Republic of China.
Bioresour Technol. 2019 Dec;293:122055. doi: 10.1016/j.biortech.2019.122055. Epub 2019 Aug 24.
In this work, amphiphilic surfactant was obtained using dehydroabietic acid from pine rosin and then pre-adsorbed with acid-pretreated bamboo residues (AP-BR) to block the residual lignin adsorption site, which is expected to improve its enzymatic digestibility. Results from cryogenic-transmission electron microscopy (Cryo-TEM) indicated amphiphilic surfactant with PEG with polymerization degree of 34 (D-34) aggregated to form worm-like micelles, which improved enzymatic hydrolysis yield of AP-BR from 24.3% to 71.9% by pre-adsorbing with 0.8 g/L. Amphiphilic surfactants pre-adsorbed on AP-BR could reduce hydrophobicity of AP-BR, adsorption affinity and adsorption capacity of lignin for cellulase from 0.51 L/g to 0.48-0.32 L/g, from 2.9 mL/mg to 1.8-1.4 mL/mg, and from 122.3 mg/g to 101.9-21.4 mg/g, respectively. These changed properties showed compelling positive contributions (R > 0.9) for free enzymes in the supernatants and sequently for final enzymatic hydrolysis yield, which was caused by blocking non-productively hydrophobic adsorption between lignin and cellulase.
在这项工作中，使用来自松香的松节油酸获得了两亲表面活性剂，然后用酸预处理的竹废料（AP-BR）预吸附以封闭残留木质素的吸附位，这有望提高其酶解性。低温透射电子显微镜（Cryo-TEM）的结果表明，具有聚合度为 34 的聚乙二醇（D-34）的两亲表面活性剂聚集形成蠕虫状胶束，通过预吸附 0.8 g/L 的 D-34，可将 AP-BR 的酶水解产率从 24.3%提高到 71.9%。预吸附在 AP-BR 上的两亲表面活性剂可降低 AP-BR 的疏水性、木质素对纤维素酶的吸附亲和力和吸附量，分别从 0.51 L/g 降至 0.48-0.32 L/g、从 2.9 mL/mg 降至 1.8-1.4 mL/mg 以及从 122.3 mg/g 降至 101.9-21.4 mg/g。这些变化的性质对上清液中游离酶表现出强烈的正贡献（R>0.9），并最终提高了酶水解产率，这是由于木质素和纤维素酶之间非生产性的疏水性吸附被阻断。
