Henry Julianne, Scandrett Lenara
Forensic Science SA, GPO Box 2790, Adelaide, South Australia 5001, Australia; College of Science and Engineering, Flinders University of South Australia, GPO Box 2100, Adelaide, South Australia 5001, Australia.
Forensic Science SA, GPO Box 2790, Adelaide, South Australia 5001, Australia.
Sci Justice. 2019 Sep;59(5):480-485. doi: 10.1016/j.scijus.2019.05.001. Epub 2019 May 8.
The ability to detect male epithelial cells deposited during digital penetration or penile penetration without ejaculation is limited by the sensitivity of the Y-STR profiling kit. In this study, the relative profiling success of the Thermofisher Yfiler® Plus kit was compared to its predecessor, AmpFlSTR Yfiler®, for 104 semen-negative sexual assault samples from casework at Forensic Science SA, Adelaide, South Australia. Yfiler Plus generated allele information in 25% more samples than Yfiler and gave a higher recovery of informative alleles in all but two samples where detectable male DNA was present. Where a profile was obtained in both kits, 92% of samples gave a higher percentage of informative loci with Yfiler Plus compared to Yfiler. Yfiler Plus also resolved DNA mixtures in 15 samples as compared to 1 sample with Yfiler. Detection of male DNA with the Quantifiler™ Trio DNA Quantification kit was shown to correlate with a successful profiling outcome with Yfiler Plus. The success of profiling with Yfiler Plus was independent of the time elapsed between the alleged offence and the sample being collected, the type of sexual penetration which occurred, and the anatomical origin of the sample.
利用Y-STR分析试剂盒检测在指交或阴茎插入但未射精过程中沉积的男性上皮细胞的能力，受到该试剂盒灵敏度的限制。在本研究中，将赛默飞世尔科技公司的Yfiler® Plus试剂盒与其前身AmpFlSTR Yfiler® 针对来自南澳大利亚阿德莱德法医科学局实际案件工作的104份精液阴性性侵犯样本进行了相对分型成功率的比较。Yfiler Plus在比Yfiler多25%的样本中生成了等位基因信息，并且在除两个存在可检测到男性DNA的样本之外的所有样本中，获得的信息量丰富的等位基因回收率更高。在两个试剂盒均获得分型结果的情况下，与Yfiler相比，92%的样本使用Yfiler Plus时具有更高比例的信息量丰富的基因座。与Yfiler仅在1个样本中解析出DNA混合物相比，Yfiler Plus在15个样本中解析出了DNA混合物。使用Quantifiler™ Trio DNA定量试剂盒检测男性DNA，结果显示与使用Yfiler Plus成功获得分型结果相关。使用Yfiler Plus进行分型的成功率与涉嫌犯罪与采集样本之间的时间间隔、发生的性侵犯类型以及样本的解剖学来源无关。
